biostudies-literaturePan LNational Institute of Allergy and Infectious DiseasesNIAID NIH HHSCrohn's & Colitis FoundationNational Natural Science Foundation of ChinaNational Postdoctoral Program for Innovative TalentsWelch Foundatione1012616https://www.ebi.ac.uk/biostudies/studies/S-EPMC11515973biostudies-literatureUnknown20(10)Respiratory syncytial virus (RSV), along with other prominent respiratory RNA viruses such as influenza and SARS-CoV-2, significantly contributes to the global incidence of respiratory tract infections. These pathogens induce the production of reactive oxygen species (ROS), which play a crucial role in the onset and progression of respiratory diseases. However, the mechanisms by which viral RNA manages ROS-induced base oxidation remain poorly understood. Here, we reveal that 8-oxo-7,8-dihydroguanine (8-oxoGua) is not merely an incidental byproduct of ROS activity but serves as a strategic adaptation of RSV RNA to maintain genetic fidelity by hijacking the 8-oxoguanine DNA glycosylase 1 (OGG1). Through RNA immunoprecipitation and next-generation sequencing, we discovered that OGG1 binding sPLoS pathogens8-Oxoguanine DNA Glycosylase1 conceals oxidized guanine in nucleoprotein-associated RNA of respiratory syncytial virus.PMC1151597332170591 and 31970686688953H-2032-2023040531900424R21 AI176297BX20200362923759AI062885P01 AI062885Zheng XHosakote YTian BGagnon MGBa XHao WBasu RSIslam ABoldogh IWang KXue YHazra TKPan Lfalse8-Oxoguanine DNA Glycosylase1 conceals oxidized guanine in nucleoprotein-associated RNA of respiratory syncytial virus.Respiratory syncytial virus (RSV), along with other prominent respiratory RNA viruses such as influenza and SARS-CoV-2, significantly contributes to the global incidence of respiratory tract infections. These pathogens induce the production of reactive oxygen species (ROS), which play a crucial role in the onset and progression of respiratory diseases. However, the mechanisms by which viral RNA manages ROS-induced base oxidation remain poorly understood. Here, we reveal that 8-oxo-7,8-dihydroguanine (8-oxoGua) is not merely an incidental byproduct of ROS activity but serves as a strategic adaptation of RSV RNA to maintain genetic fidelity by hijacking the 8-oxoguanine DNA glycosylase 1 (OGG1). Through RNA immunoprecipitation and next-generation sequencing, we discovered that OGG1 binding s2024-01-01T00:00:00Z2024 Oct2026-07-06T03:09:57.103Z2025-04-06T14:23:03.264ZS-EPMC115159733941314310.1371/journal.ppat.1012616