biostudies-literatureLi MChengdu Women and Children&apos;s Hospital Talent Plane0312178https://www.ebi.ac.uk/biostudies/studies/S-EPMC11515993biostudies-literatureUnknown19(10)<h4>Purpose</h4>Wilms' tumor (WT), also known as nephroblastoma, is the predominant form of primary malignant renal cancer. The unfavorable prognoses linked to anaplastic nephroblastoma and recurrent nephroblastoma emphasize the crucial requirement for the exploration of innovative treatment modalities for WT.<h4>Methods</h4>Our study conducted one-way Cox regression and Kaplan-Meier analyses using TARGET-WT nephroblastoma data to identify differentially expressed genes in nephroblastoma and evaluate their prognostic relevance. Utilizing the Connectivity Map database, ZSTK474 emerged as a viable therapeutic option for WT. The effect of ZSTK474 on WT and related underlying mechanisms were further investigated through in vitro and in vivo investigations.<h4>Results</h4>The in vivo experimentPloS oneZSTK474 targeting PIK3R3 inhibits the Wilms' tumor through G0 / G1 phase arrest.PMC11515993No.YC2022017Liu JHe DMi TZhang ZWang JWang ZLi MZhanghuang CJin LWu XfalseZSTK474 targeting PIK3R3 inhibits the Wilms' tumor through G0 / G1 phase arrest.<h4>Purpose</h4>Wilms' tumor (WT), also known as nephroblastoma, is the predominant form of primary malignant renal cancer. The unfavorable prognoses linked to anaplastic nephroblastoma and recurrent nephroblastoma emphasize the crucial requirement for the exploration of innovative treatment modalities for WT.<h4>Methods</h4>Our study conducted one-way Cox regression and Kaplan-Meier analyses using TARGET-WT nephroblastoma data to identify differentially expressed genes in nephroblastoma and evaluate their prognostic relevance. Utilizing the Connectivity Map database, ZSTK474 emerged as a viable therapeutic option for WT. The effect of ZSTK474 on WT and related underlying mechanisms were further investigated through in vitro and in vivo investigations.<h4>Results</h4>The in vivo experiment2024-01-01T00:00:00Z20242026-07-07T03:17:26.948Z2025-04-04T00:29:57.736ZS-EPMC115159933946676310.1371/journal.pone.0312178