biostudies-literatureHe WNational Natural Science Foundation of China2921-2931https://www.ebi.ac.uk/biostudies/studies/S-EPMC11519176biostudies-literatureUnknown63(8)<h4>Purpose</h4>This study aims to evaluate a combined effect of dietary folic acid intake, multiple genetic polymorphisms in folate metabolism, and other environmental factors on red blood cell (RBC) folate concentration in pregnancy-preparing population.<h4>Methods</h4>519 pregnancy-preparing subjects (260 couples) were investigated. Dietary intake was measured by 3-day dietary recalls. 13 Single Nucleotide polymorphisms (SNPs) reported in association with one-carbon metabolism including the methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C were genotyped. RBC folate concentration was measured using chemiluminescence assay. Hierarchical regression was applied for covariate selection. Factors showed significance(p < 0.0125) on RBC folate level was included for prediction model European journal of nutritionDietary folic acid intake, 13 genetic variants and other factors with red blood cell folate concentration in pregnancy-preparing population.PMC115191768237358482070323Dou YHe YHe WZhang YChen XYan WfalseDietary folic acid intake, 13 genetic variants and other factors with red blood cell folate concentration in pregnancy-preparing population.<h4>Purpose</h4>This study aims to evaluate a combined effect of dietary folic acid intake, multiple genetic polymorphisms in folate metabolism, and other environmental factors on red blood cell (RBC) folate concentration in pregnancy-preparing population.<h4>Methods</h4>519 pregnancy-preparing subjects (260 couples) were investigated. Dietary intake was measured by 3-day dietary recalls. 13 Single Nucleotide polymorphisms (SNPs) reported in association with one-carbon metabolism including the methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C were genotyped. RBC folate concentration was measured using chemiluminescence assay. Hierarchical regression was applied for covariate selection. Factors showed significance(p < 0.0125) on RBC folate level was included for prediction model 2024-01-01T00:00:00Z2024 Dec2026-07-15T17:37:58.284Z2025-04-04T00:26:34.999ZS-EPMC115191763915312510.1007/s00394-024-03474-z