biostudies-literatureWang Pthe Open Project of state key laboratory of military stomatologyNational Natural Science Foundation of China66https://www.ebi.ac.uk/biostudies/studies/S-EPMC11519219biostudies-literatureUnknown35(1)An acellular bovine bone matrix modified to release Magnesium ions (Mg²⁺) (ABBM-Mg) was prepared and evaluated for its potential in osteogenesis and mandibular defect repair. Mg²⁺ was incorporated into ABBM using an ion exchange method. The microstructure and mechanical properties of both ABBM and ABBM-Mg were analyzed using SEM and a biomechanical testing machine. Cytocompatibility, cell adhesion, and osteogenic differentiation were assessed using various methods including CCK-8, Live/Dead staining, SEM, ALP staining, and qPCR analysis in MC3T3-E1 cells. Additionally, a mandibular defect model in rats was established. The bone defect repair outcomes were evaluated using Micro-CT, histological HE staining, and Masson staining. The study showed that mineralization contJournal of materials science. Materials in medicineEnhancing osteogenesis and mandibular defect repair with magnesium-modified acellular bovine bone matrix.PMC11519219822048202019KA01, 2019KB01Ge RWang PHan LHu XLi BLi LXu GYu KfalseEnhancing osteogenesis and mandibular defect repair with magnesium-modified acellular bovine bone matrix.An acellular bovine bone matrix modified to release Magnesium ions (Mg²⁺) (ABBM-Mg) was prepared and evaluated for its potential in osteogenesis and mandibular defect repair. Mg²⁺ was incorporated into ABBM using an ion exchange method. The microstructure and mechanical properties of both ABBM and ABBM-Mg were analyzed using SEM and a biomechanical testing machine. Cytocompatibility, cell adhesion, and osteogenic differentiation were assessed using various methods including CCK-8, Live/Dead staining, SEM, ALP staining, and qPCR analysis in MC3T3-E1 cells. Additionally, a mandibular defect model in rats was established. The bone defect repair outcomes were evaluated using Micro-CT, histological HE staining, and Masson staining. The study showed that mineralization cont2024-01-01T00:00:00Z2024 Oct2025-04-04T01:08:43.078Z2025-04-04T01:08:43.078ZS-EPMC115192193946646810.1007/s10856-024-06835-6