biostudies-literatureUnknown25(1)Chi Z<h4>Background</h4>Mongolian cattle are local breeds in northern China with excellent adaptability to harsh environmental conditions. Adipose tissues play essential roles in tolerance to cold and disease, but the associated cellular and molecular mechanisms are unclear.<h4>Methods</h4>Single-nucleus RNA sequencing (snRNA-seq) was performed on the adipose tissues from the subcutaneous (SAT), greater omentum (OAT) and perirenal (PAT) of 3 healthy cattle. The adipogenic trajectory was analyzed, and the functional roles of gene of interest were verified in vitro.<h4>Results</h4>There were different cell subpopulations in adipose tissues. The lipid-deposition adipocytes identified by the PTGER3 marker exhibited outstanding characteristics in SAT. In PAT and OAT, aldosterone was expressed to proBMC genomics999https://www.ebi.ac.uk/biostudies/studies/S-EPMC11520132biostudies-literaturesnRNA-seq of adipose tissues reveals the potential cellular and molecular mechanisms of cold and disease resistance in Mongolian cattle.PMC11520132Yang HQiqi GHe JZhang JSui ZFan RMengke BHerrid MJia QRen HAi DJin CZhu LWuri NAierqing SWuli JChi ZfalsesnRNA-seq of adipose tissues reveals the potential cellular and molecular mechanisms of cold and disease resistance in Mongolian cattle.<h4>Background</h4>Mongolian cattle are local breeds in northern China with excellent adaptability to harsh environmental conditions. Adipose tissues play essential roles in tolerance to cold and disease, but the associated cellular and molecular mechanisms are unclear.<h4>Methods</h4>Single-nucleus RNA sequencing (snRNA-seq) was performed on the adipose tissues from the subcutaneous (SAT), greater omentum (OAT) and perirenal (PAT) of 3 healthy cattle. The adipogenic trajectory was analyzed, and the functional roles of gene of interest were verified in vitro.<h4>Results</h4>There were different cell subpopulations in adipose tissues. The lipid-deposition adipocytes identified by the PTGER3 marker exhibited outstanding characteristics in SAT. In PAT and OAT, aldosterone was expressed to pro2024-01-01T00:00:00Z2024 Oct2026-07-15T15:32:31.433Z2025-04-19T18:11:26.19ZS-EPMC115201323944889910.1186/s12864-024-10913-y