biostudies-literatureGomez-Mejia ASwiss National Science Foundatione0043024https://www.ebi.ac.uk/biostudies/studies/S-EPMC11520294biostudies-literatureUnknown9(10)The human pathobiont <i>Streptococcus pyogenes</i> forms biofilms and causes infections, such as pharyngotonsillitis and necrotizing fasciitis. Bacterial biofilms are more resilient to antibiotic treatment, and new therapeutic strategies are needed to control biofilm-associated infections, such as recurrent pharyngotonsillitis. <i>Lactiplantibacillus plantarum</i> and <i>Lacticaseibacillus rhamnosus</i> are two bacterial commensals used for their probiotic properties. This study aimed to elucidate the anti-biofilm properties of <i>L. plantarum</i> and <i>L. rhamnosus</i> cell-free supernatants (LPSN and LRSN, respectively) on <i>S. pyogenes</i> biofilms grown <i>in vitro</i> in supplemented minimal medium. When planktonic or biofilm <i>S. pyogenes</i> were exposed to LPSN or LRSN<i>, S. pymSphereInhibition of <i>Streptococcus pyogenes</i> biofilm by <i>Lactiplantibacillus plantarum</i> and <i>Lacticaseibacillus rhamnosus</i>.PMC11520294169962204343310030Mairpady Shambat SGomez-Mejia AOrlietti MZinkernagel ASTarnutzer AfalseInhibition of <i>Streptococcus pyogenes</i> biofilm by <i>Lactiplantibacillus plantarum</i> and <i>Lacticaseibacillus rhamnosus</i>.The human pathobiont <i>Streptococcus pyogenes</i> forms biofilms and causes infections, such as pharyngotonsillitis and necrotizing fasciitis. Bacterial biofilms are more resilient to antibiotic treatment, and new therapeutic strategies are needed to control biofilm-associated infections, such as recurrent pharyngotonsillitis. <i>Lactiplantibacillus plantarum</i> and <i>Lacticaseibacillus rhamnosus</i> are two bacterial commensals used for their probiotic properties. This study aimed to elucidate the anti-biofilm properties of <i>L. plantarum</i> and <i>L. rhamnosus</i> cell-free supernatants (LPSN and LRSN, respectively) on <i>S. pyogenes</i> biofilms grown <i>in vitro</i> in supplemented minimal medium. When planktonic or biofilm <i>S. pyogenes</i> were exposed to LPSN or LRSN<i>, S. py2024-01-01T00:00:00Z2024 Oct2026-07-15T14:50:30.511Z2025-04-04T01:02:42.366ZS-EPMC115202943936083910.1128/msphere.00430-24