{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wu Y"],"funding":["Guizhou Provincial High-level Innovative Talents Project","National Natural Science Foundation of China"],"pagination":["1010"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11520388"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(1)"],"pubmed_abstract":["The quality of Recombination signal sequences (RSSs), location, and genetics of mammalian V, D, and J genes synergistically affect the recombination frequency of genes; however, the specific regulatory mechanism and efficiency have not been elucidated. By taking advantage of single-cell RNA-sequencing (scRNA-seq) and high-throughput sequencing (HTS) to investigate V(D)J rearrangement characteristics in the CDR3 repertoire, we found that the distal and proximal V genes (or J genes) \"to D\" gene were involved in rearrangement significantly more frequently than the middle V genes (or J genes) in the TRB locus among various species, including Primates (human and rhesus monkey), Rodentia (BALB/c, C57BL/6, and Kunming mice), Artiodactyla (buffalo), and Chiroptera (Rhinolophus affinis). The RSS qu"],"journal":["BMC genomics"],"pubmed_title":["HTS and scRNA-seq revealed that the location and RSS quality of the mammalian TRBV and TRBJ genes impact biased rearrangement."],"pmcid":["PMC11520388"],"funding_grant_id":["No. (2018) 5637","82160279"],"pubmed_authors":["Ma Q","Li J","Ma L","Yao X","Wu F","Zhou H","Gong Y","Wu Y"],"additional_accession":[]},"is_claimable":false,"name":"HTS and scRNA-seq revealed that the location and RSS quality of the mammalian TRBV and TRBJ genes impact biased rearrangement.","description":"The quality of Recombination signal sequences (RSSs), location, and genetics of mammalian V, D, and J genes synergistically affect the recombination frequency of genes; however, the specific regulatory mechanism and efficiency have not been elucidated. By taking advantage of single-cell RNA-sequencing (scRNA-seq) and high-throughput sequencing (HTS) to investigate V(D)J rearrangement characteristics in the CDR3 repertoire, we found that the distal and proximal V genes (or J genes) \"to D\" gene were involved in rearrangement significantly more frequently than the middle V genes (or J genes) in the TRB locus among various species, including Primates (human and rhesus monkey), Rodentia (BALB/c, C57BL/6, and Kunming mice), Artiodactyla (buffalo), and Chiroptera (Rhinolophus affinis). The RSS qu","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2026-07-15T14:42:16.891Z","creation":"2025-04-04T22:47:47.178Z"},"accession":"S-EPMC11520388","cross_references":{"pubmed":["39472808"],"doi":["10.1186/s12864-024-10887-x"]}}