{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chengmin Z"],"funding":["Sichuan Provincial Foundation of Science and Technology"],"pagination":["e25106"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11520935"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["38(19-20)"],"pubmed_abstract":["<h4>Objective</h4>To compare the application of different treatments in the diagnosis of melanoma with severe pigment interference, to solve the problem of pigment interference with immunohistochemical interpretation.<h4>Methods</h4>The pigment-rich melanomas were first depigmented with potassium permanganate using a concentration gradient (0.1%, 0.5%, 1%) and a time gradient (1, 5, 10, 15, 30 min, 6 h), and the optimal concentration and time were found. Then, 12 cases of pigment-rich melanoma tissues were collected, and the tissues were stained with diaminobenzidine (DAB), alkaline phosphatase-fast red (AP red), multiplex immunofluorescence (MIF), and 3-amino-9-ethylcarbazole (AEC), and ferrous sulfate, comparing different methods, positive expression of HMB45, MelanA, S100, SOX10, ki67.<"],"journal":["Journal of clinical laboratory analysis"],"pubmed_title":["Application of Different Staining Methods in the Diagnosis of Pigment-Rich Melanoma."],"pmcid":["PMC11520935"],"funding_grant_id":["2023NSFSC0626"],"pubmed_authors":["Jun H","Jiayu L","Yang L","Zhou Y","Chengmin Z"],"additional_accession":[]},"is_claimable":false,"name":"Application of Different Staining Methods in the Diagnosis of Pigment-Rich Melanoma.","description":"<h4>Objective</h4>To compare the application of different treatments in the diagnosis of melanoma with severe pigment interference, to solve the problem of pigment interference with immunohistochemical interpretation.<h4>Methods</h4>The pigment-rich melanomas were first depigmented with potassium permanganate using a concentration gradient (0.1%, 0.5%, 1%) and a time gradient (1, 5, 10, 15, 30 min, 6 h), and the optimal concentration and time were found. Then, 12 cases of pigment-rich melanoma tissues were collected, and the tissues were stained with diaminobenzidine (DAB), alkaline phosphatase-fast red (AP red), multiplex immunofluorescence (MIF), and 3-amino-9-ethylcarbazole (AEC), and ferrous sulfate, comparing different methods, positive expression of HMB45, MelanA, S100, SOX10, ki67.<","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2025-04-05T09:12:36.568Z","creation":"2025-04-05T09:12:36.568Z"},"accession":"S-EPMC11520935","cross_references":{"pubmed":["39315764"],"doi":["10.1002/jcla.25106"]}}