biostudies-literatureChengmin ZSichuan Provincial Foundation of Science and Technologye25106https://www.ebi.ac.uk/biostudies/studies/S-EPMC11520935biostudies-literatureUnknown38(19-20)<h4>Objective</h4>To compare the application of different treatments in the diagnosis of melanoma with severe pigment interference, to solve the problem of pigment interference with immunohistochemical interpretation.<h4>Methods</h4>The pigment-rich melanomas were first depigmented with potassium permanganate using a concentration gradient (0.1%, 0.5%, 1%) and a time gradient (1, 5, 10, 15, 30 min, 6 h), and the optimal concentration and time were found. Then, 12 cases of pigment-rich melanoma tissues were collected, and the tissues were stained with diaminobenzidine (DAB), alkaline phosphatase-fast red (AP red), multiplex immunofluorescence (MIF), and 3-amino-9-ethylcarbazole (AEC), and ferrous sulfate, comparing different methods, positive expression of HMB45, MelanA, S100, SOX10, ki67.<Journal of clinical laboratory analysisApplication of Different Staining Methods in the Diagnosis of Pigment-Rich Melanoma.PMC115209352023NSFSC0626Jun HJiayu LYang LZhou YChengmin ZfalseApplication of Different Staining Methods in the Diagnosis of Pigment-Rich Melanoma.<h4>Objective</h4>To compare the application of different treatments in the diagnosis of melanoma with severe pigment interference, to solve the problem of pigment interference with immunohistochemical interpretation.<h4>Methods</h4>The pigment-rich melanomas were first depigmented with potassium permanganate using a concentration gradient (0.1%, 0.5%, 1%) and a time gradient (1, 5, 10, 15, 30 min, 6 h), and the optimal concentration and time were found. Then, 12 cases of pigment-rich melanoma tissues were collected, and the tissues were stained with diaminobenzidine (DAB), alkaline phosphatase-fast red (AP red), multiplex immunofluorescence (MIF), and 3-amino-9-ethylcarbazole (AEC), and ferrous sulfate, comparing different methods, positive expression of HMB45, MelanA, S100, SOX10, ki67.<2024-01-01T00:00:00Z2024 Oct2025-04-05T09:12:36.568Z2025-04-05T09:12:36.568ZS-EPMC115209353931576410.1002/jcla.25106