{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gao X"],"funding":["National Natural Science Foundation of China (National Science Foundation of China)"],"pagination":["9313"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11522295"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["Tumor heterogeneity and tumor evolution contribute to cancer treatment failure. To understand how selective pressures drive heterogeneous tumor evolution, it would be useful to image multiple important components and tumor subclones in vivo. We propose a supercontinuum-tailoring two-photon microscope (SCT-TPM) and realize simultaneous observation of nine fluorophores with a single light beam, breaking through the 'color barrier' of intravital two-photon fluorescence imaging. It achieves excitation multiplexing only by modulating the phase of fiber supercontinuum (SC), allowing to capture rapid events of multiple targets with maintaining precise spatial alignment. We employ SCT-TPM to visualize the spatiotemporal dynamics of heterogeneous tumor evolution under host immune surveillance, part"],"journal":["Nature communications"],"pubmed_title":["Supercontinuum-tailoring multicolor imaging reveals spatiotemporal dynamics of heterogeneous tumor evolution."],"pmcid":["PMC11522295"],"funding_grant_id":["62475063"],"pubmed_authors":["Hou Z","Yang J","Yang L","Liu Q","Zhou Y","Qi S","Liu Z","Luo Q","Chen Z","Zhang Z","Huang X","Fu L","Gao X"],"additional_accession":[]},"is_claimable":false,"name":"Supercontinuum-tailoring multicolor imaging reveals spatiotemporal dynamics of heterogeneous tumor evolution.","description":"Tumor heterogeneity and tumor evolution contribute to cancer treatment failure. To understand how selective pressures drive heterogeneous tumor evolution, it would be useful to image multiple important components and tumor subclones in vivo. We propose a supercontinuum-tailoring two-photon microscope (SCT-TPM) and realize simultaneous observation of nine fluorophores with a single light beam, breaking through the 'color barrier' of intravital two-photon fluorescence imaging. It achieves excitation multiplexing only by modulating the phase of fiber supercontinuum (SC), allowing to capture rapid events of multiple targets with maintaining precise spatial alignment. We employ SCT-TPM to visualize the spatiotemporal dynamics of heterogeneous tumor evolution under host immune surveillance, part","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2026-07-15T17:43:01.245Z","creation":"2025-04-04T13:20:30.652Z"},"accession":"S-EPMC11522295","cross_references":{"pubmed":["39472437"],"doi":["10.1038/s41467-024-53697-1"]}}