{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Simwela NV"],"funding":["U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases","NIAID NIH HHS","U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID)","Mueller Health Foundation","U.S. Department of Health & Human Services | NIH | NIH Office of the Director","NIH HHS","U.S. Department of Health & Human Services | NIH | NIH Office of the Director (OD)"],"pagination":["9322"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11522665"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["The eukaryotic Glucose Induced Degradation/C-Terminal to LisH (GID/CTLH) complex is a highly conserved E3 ubiquitin ligase involved in a broad range of biological processes. However, a role of this complex in host anti-microbial defenses has not been described. We exploited Mycobacterium tuberculosis (Mtb) induced cytotoxicity in macrophages in a FACS based CRISPR genetic screen to identify host determinants of intracellular Mtb growth restriction. Our screen identified 5 (GID8, YPEL5, WDR26, UBE2H, MAEA) of the 12 predicted members of the GID/CTLH complex as determinants of intracellular growth of both Mtb and Salmonella serovar Typhimurium. We show that the anti-microbial properties of the GID/CTLH complex knockout macrophages are mediated by enhanced GABAergic signaling, activated AMPK,"],"journal":["Nature communications"],"pubmed_title":["Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth."],"pmcid":["PMC11522665"],"funding_grant_id":["S10 OD032135","AI155319","R01 AI155319","U19 AI162598","R01 AI172433","OD032135","AI162598","AI172433","T32AI007349","T32 AI007349"],"pubmed_authors":["Simwela NV","Sassetti CM","Jaecklein E","Johnston L","Altier C","Bitar PP","Russell DG"],"additional_accession":[]},"is_claimable":false,"name":"Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth.","description":"The eukaryotic Glucose Induced Degradation/C-Terminal to LisH (GID/CTLH) complex is a highly conserved E3 ubiquitin ligase involved in a broad range of biological processes. However, a role of this complex in host anti-microbial defenses has not been described. We exploited Mycobacterium tuberculosis (Mtb) induced cytotoxicity in macrophages in a FACS based CRISPR genetic screen to identify host determinants of intracellular Mtb growth restriction. Our screen identified 5 (GID8, YPEL5, WDR26, UBE2H, MAEA) of the 12 predicted members of the GID/CTLH complex as determinants of intracellular growth of both Mtb and Salmonella serovar Typhimurium. We show that the anti-microbial properties of the GID/CTLH complex knockout macrophages are mediated by enhanced GABAergic signaling, activated AMPK,","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2025-04-05T09:12:24.934Z","creation":"2025-04-05T09:12:24.934Z"},"accession":"S-EPMC11522665","cross_references":{"pubmed":["39472457"],"doi":["10.1038/s41467-024-53637-z"]}}