biostudies-literatureGilbert SJMedical Research Council CanadaVersus ArthritisJGW Patterson FoundationMedical Research CouncilNewcastle UniversityVivensa Foundationdmm050583https://www.ebi.ac.uk/biostudies/studies/S-EPMC11524441biostudies-literatureUnknown17(10)Animal models of post-traumatic osteoarthritis (PTOA) recapitulate the pathological changes observed in human PTOA. Here, skeletally mature C57Bl6 mice were subjected to either rapid-onset non-surgical mechanical rupture of the anterior cruciate ligament (ACL) or to surgical destabilisation of the medial meniscus (DMM). Transcriptome profiling of micro-dissected cartilage at day 7 or day 42 following ACL or DMM procedure, respectively, showed that the two models were comparable and highly correlative. Gene ontology (GO) enrichment analysis identified similarly enriched pathways that were overrepresented by anabolic terms. To address the transcriptome changes more completely in the ACL model, we also performed small RNA sequencing, describing the first microRNA profile of this model. miR-19Disease models & mechanismsComparative transcriptomic analysis of articular cartilage of post-traumatic osteoarthritis models.PMC115244411846122043JXR 10641JXR 10641, MR/P020941/1R476/0516Pirog KAYoung DABlain EJSoul JLin HCoxhead JHao YBarter MJGilbert SJPatel KfalseComparative transcriptomic analysis of articular cartilage of post-traumatic osteoarthritis models.Animal models of post-traumatic osteoarthritis (PTOA) recapitulate the pathological changes observed in human PTOA. Here, skeletally mature C57Bl6 mice were subjected to either rapid-onset non-surgical mechanical rupture of the anterior cruciate ligament (ACL) or to surgical destabilisation of the medial meniscus (DMM). Transcriptome profiling of micro-dissected cartilage at day 7 or day 42 following ACL or DMM procedure, respectively, showed that the two models were comparable and highly correlative. Gene ontology (GO) enrichment analysis identified similarly enriched pathways that were overrepresented by anabolic terms. To address the transcriptome changes more completely in the ACL model, we also performed small RNA sequencing, describing the first microRNA profile of this model. miR-192024-01-01T00:00:00Z2024 Oct2026-06-01T09:55:24.075Z2025-04-05T09:31:18.474ZS-EPMC115244413931405810.1242/dmm.050583