biostudies-literatureUnknown15Yuan S<h4>Introduction</h4>Microalgae, known for their adaptability to extreme environments, are important for basic research and industrial applications. <i>Euglena</i>, unique for its lack of a cell wall, has garnered attention due to its versatility and the presence of bioactive compounds. Despite its potential, few studies have focused on <i>Euglena</i>'s cold adaptation mechanisms.<h4>Methods</h4>This study investigates the cold adaptation mechanisms of <i>Euglena gracilis</i>, a microalga found in highly diverse environmental habitats, by comparing its growth, photosynthetic performance, and physiological and biochemical responses under two low-temperature cultivation modes: pre-adaptation to 16°C followed by exposure to 4°C (PreC) and direct exposure to 4°C (DirC).<h4>Results and discussiFrontiers in microbiology1465351https://www.ebi.ac.uk/biostudies/studies/S-EPMC11524907biostudies-literatureEnhanced cold tolerance mechanisms in &lt;i&gt;Euglena gracilis&lt;/i&gt;: comparative analysis of pre-adaptation and direct low-temperature exposure.PMC11524907Yuan SZhang DLi CChen ZFu WYao RWang JDu MfalseEnhanced cold tolerance mechanisms in &lt;i&gt;Euglena gracilis&lt;/i&gt;: comparative analysis of pre-adaptation and direct low-temperature exposure.<h4>Introduction</h4>Microalgae, known for their adaptability to extreme environments, are important for basic research and industrial applications. <i>Euglena</i>, unique for its lack of a cell wall, has garnered attention due to its versatility and the presence of bioactive compounds. Despite its potential, few studies have focused on <i>Euglena</i>'s cold adaptation mechanisms.<h4>Methods</h4>This study investigates the cold adaptation mechanisms of <i>Euglena gracilis</i>, a microalga found in highly diverse environmental habitats, by comparing its growth, photosynthetic performance, and physiological and biochemical responses under two low-temperature cultivation modes: pre-adaptation to 16°C followed by exposure to 4°C (PreC) and direct exposure to 4°C (DirC).<h4>Results and discussi2024-01-01T00:00:00Z20242025-04-04T02:01:19.664Z2025-04-04T02:01:19.664ZS-EPMC115249073948375910.3389/fmicb.2024.1465351