biostudies-literatureYasui TMoonshot Research and Development ProgramJapan Agency for Medical Research and DevelopmentAIP Network LaboratoryJapan Society for the Promotion of ScienceNew Energy and Industrial Technology Development Organization17145-17153https://www.ebi.ac.uk/biostudies/studies/S-EPMC11525924biostudies-literatureUnknown96(43)Multiple microRNAs encapsulated in extracellular vesicles (EVs) including exosomes, unique subtypes of EVs, differ in healthy and cancer groups of people, and they represent a warning sign for various cancer scenarios. Since all EVs in blood cannot be transferred from donor to recipient cells during a single blood circulation, kidney filtration could pass some untransferred EVs from blood to urine. Previously, we reported on the ability of zinc oxide nanowires to capture EVs based on surface charge and hydrogen bonding; these nanowires extracted massive numbers of microRNAs in urine, seeking cancer-related microRNAs through statistical analysis. Here, we report on the scalability of the nanowire performance capability to comprehensively capture EVs, including exosomes, in urine, extract miAnalytical chemistryEarly Cancer Detection via Multi-microRNA Profiling of Urinary Exosomes Captured by Nanowires.PMC1152592422zf0127004s0902JPNP20004JP21he2302007JPMJCR23U124H00792JP22zf0127009Ichikawa YIida MNatsume AYanagida TKitano YShimada TYasui TKawai TOchiya TAoki KTakayama KWashio TBaba YMizunuma MChattrairat KNaganawa TNagashima KfalseEarly Cancer Detection via Multi-microRNA Profiling of Urinary Exosomes Captured by Nanowires.Multiple microRNAs encapsulated in extracellular vesicles (EVs) including exosomes, unique subtypes of EVs, differ in healthy and cancer groups of people, and they represent a warning sign for various cancer scenarios. Since all EVs in blood cannot be transferred from donor to recipient cells during a single blood circulation, kidney filtration could pass some untransferred EVs from blood to urine. Previously, we reported on the ability of zinc oxide nanowires to capture EVs based on surface charge and hydrogen bonding; these nanowires extracted massive numbers of microRNAs in urine, seeking cancer-related microRNAs through statistical analysis. Here, we report on the scalability of the nanowire performance capability to comprehensively capture EVs, including exosomes, in urine, extract mi2024-01-01T00:00:00Z2024 Oct2025-04-26T17:10:45.937Z2025-04-06T15:23:55.058ZS-EPMC115259243942233410.1021/acs.analchem.4c02488