{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Coughlin BA"],"pubmed_abstract":["<h4>Introduction</h4>Inflammation and cell death play an important role in the pathogenesis of diabetic retinopathy. Previously we observed sustained activation of pro-inflammatory caspase-1 in retinas of diabetic animals and patients. In this study, we aimed to look at mechanisms underlying chronic caspase-1 activation <i>in vitro</i> and <i>in vivo</i>.<h4>Methods</h4>Non-diabetic and diabetic wild type and IL-1 receptor (IL-1R1) knockout mice were used for <i>in vivo</i> experiments. Diabetes was induced using STZ (streptozotocin). Human Müller cells were used for <i>in vitro</i> studies. Cells were treated with either 5 mM or 25 mM glucose or interleukin-1beta (IL-1β) in the presence or absence of IL-1 receptor antagonist (IL-1ra) or siRNA against RIP2 (receptor interacting protein-2) "],"journal":["Frontiers in cell and developmental biology"],"pagination":["1467799"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11525982"],"repository":["biostudies-literature"],"pubmed_title":["Interleukin-1 receptor-dependent and -independent caspase-1 activity in retinal cells mediated by receptor interacting protein 2."],"pmcid":["PMC11525982"],"pubmed_authors":["Christian B","Trombley B","Coughlin BA","Mohr S"],"additional_accession":[]},"is_claimable":false,"name":"Interleukin-1 receptor-dependent and -independent caspase-1 activity in retinal cells mediated by receptor interacting protein 2.","description":"<h4>Introduction</h4>Inflammation and cell death play an important role in the pathogenesis of diabetic retinopathy. Previously we observed sustained activation of pro-inflammatory caspase-1 in retinas of diabetic animals and patients. In this study, we aimed to look at mechanisms underlying chronic caspase-1 activation <i>in vitro</i> and <i>in vivo</i>.<h4>Methods</h4>Non-diabetic and diabetic wild type and IL-1 receptor (IL-1R1) knockout mice were used for <i>in vivo</i> experiments. Diabetes was induced using STZ (streptozotocin). Human Müller cells were used for <i>in vitro</i> studies. Cells were treated with either 5 mM or 25 mM glucose or interleukin-1beta (IL-1β) in the presence or absence of IL-1 receptor antagonist (IL-1ra) or siRNA against RIP2 (receptor interacting protein-2) ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024","modification":"2025-04-21T16:39:16.066Z","creation":"2025-04-21T16:39:16.066Z"},"accession":"S-EPMC11525982","cross_references":{"pubmed":["39483336"],"doi":["10.3389/fcell.2024.1467799"]}}