{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wilkes RA"],"funding":["Division of Chemistry","Bioenergy Technologies Office"],"pagination":["19338-19352"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11526368"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["58(43)"],"pubmed_abstract":["<i>Comamonadaceae</i> bacteria are enriched on poly(ethylene terephthalate) (PET) microplastics in wastewaters and urban rivers, but the PET-degrading mechanisms remain unclear. Here, we investigated these mechanisms with <i>Comamonas testosteroni</i>KF-1, a wastewater isolate, by combining microscopy, spectroscopy, proteomics, protein modeling, and genetic engineering. Compared to minor dents on PET films, scanning electron microscopy revealed significant fragmentation of PET pellets, resulting in a 3.5-fold increase in the abundance of small nanoparticles (<100 nm) during 30-day cultivation. Infrared spectroscopy captured primarily hydrolytic cleavage in the fragmented pellet particles. Solution analysis further demonstrated double hydrolysis of a PET oligomer, bis(2-hydroxyethyl) tereph"],"journal":["Environmental science & technology"],"pubmed_title":["Mechanisms of Polyethylene Terephthalate Pellet Fragmentation into Nanoplastics and Assimilable Carbons by Wastewater <i>Comamonas</i>."],"pmcid":["PMC11526368"],"funding_grant_id":["DE-AC05-00OR22725","CHE-2109097"],"pubmed_authors":["Zhou N","Wilson RS","Guss AM","Aryal O","Aristilde L","Wilkes RA","Carroll AL","Kapoor A","Zhang L","Teitel KP","Castaneda E","Waldbauer JR"],"additional_accession":[]},"is_claimable":false,"name":"Mechanisms of Polyethylene Terephthalate Pellet Fragmentation into Nanoplastics and Assimilable Carbons by Wastewater <i>Comamonas</i>.","description":"<i>Comamonadaceae</i> bacteria are enriched on poly(ethylene terephthalate) (PET) microplastics in wastewaters and urban rivers, but the PET-degrading mechanisms remain unclear. Here, we investigated these mechanisms with <i>Comamonas testosteroni</i>KF-1, a wastewater isolate, by combining microscopy, spectroscopy, proteomics, protein modeling, and genetic engineering. Compared to minor dents on PET films, scanning electron microscopy revealed significant fragmentation of PET pellets, resulting in a 3.5-fold increase in the abundance of small nanoparticles (<100 nm) during 30-day cultivation. Infrared spectroscopy captured primarily hydrolytic cleavage in the fragmented pellet particles. Solution analysis further demonstrated double hydrolysis of a PET oligomer, bis(2-hydroxyethyl) tereph","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2026-06-01T09:55:20.414Z","creation":"2025-04-06T09:32:21.049Z"},"accession":"S-EPMC11526368","cross_references":{"pubmed":["39360733"],"doi":["10.1021/acs.est.4c06645"]}}