biostudies-literatureUnknown16(1)Zhao J<h4>Background</h4>Circular RNAs (circRNAs) have emerged as a prominent class of covalently closed single-stranded RNA molecules that exhibit tissue-specific expression and potential as biomarkers in extracellular vesicles (EVs) derived from liquid biopsies. Still, their characteristics and applications in EVs remain to be unveiled.<h4>Methods</h4>We performed a comprehensive analysis of EV-derived circRNAs (EV-circRNAs) using transcriptomics data obtained from 1082 human body fluids, including plasma, urine, cerebrospinal fluid (CSF), and bile. Our validation strategy utilized RT-qPCR and RNA immunoprecipitation assays, complemented by computational techniques for analyzing EV-circRNA features and RNA-binding protein interactions.<h4>Results</h4>We identified 136,327 EV-circRNAs from variGenome medicine126https://www.ebi.ac.uk/biostudies/studies/S-EPMC11526565biostudies-literatureCircular RNA landscape in extracellular vesicles from human biofluids.PMC11526565Lai HLi YHuang SFang ZHu JZhang HLi QShen YZhao JYu HfalseCircular RNA landscape in extracellular vesicles from human biofluids.<h4>Background</h4>Circular RNAs (circRNAs) have emerged as a prominent class of covalently closed single-stranded RNA molecules that exhibit tissue-specific expression and potential as biomarkers in extracellular vesicles (EVs) derived from liquid biopsies. Still, their characteristics and applications in EVs remain to be unveiled.<h4>Methods</h4>We performed a comprehensive analysis of EV-derived circRNAs (EV-circRNAs) using transcriptomics data obtained from 1082 human body fluids, including plasma, urine, cerebrospinal fluid (CSF), and bile. Our validation strategy utilized RT-qPCR and RNA immunoprecipitation assays, complemented by computational techniques for analyzing EV-circRNA features and RNA-binding protein interactions.<h4>Results</h4>We identified 136,327 EV-circRNAs from vari2024-01-01T00:00:00Z2024 Oct2026-07-16T03:06:48.361Z2025-04-06T09:33:21.594ZS-EPMC115265653948278310.1186/s13073-024-01400-w