biostudies-literatureUnknownBa ANIAID NIH HHSFIC NIH HHSThe <i>Plasmodium falciparum</i> cysteine-rich protective antigen (PfCyRPA) is a promising target as a next-generation blood-stage malaria vaccine and together with PCRCR complex members, the reticulocyte binding-like homologous protein 5 (PfRh5) and the Rh5-interacting protein (PfRipr), are currently being evaluated in clinical trials. PfCyRPA is essential for merozoite invasion and appears to be highly conserved within the <i>P. falciparum</i> parasite populations. Here, we used a targeted deep amplicon next-generation sequencing approach to assess the breadth of PfCyRPA genetic diversity in 95 <i>P. falciparum</i> clinical isolates from Kédougou, an area with a high seasonal malaria transmission in Senegal. Our data show the dominant prevalence of PfCyRPA wild type reference allele, whimedRxiv : the preprint server for health sciences2024.10.13.24305808https://www.ebi.ac.uk/biostudies/studies/S-EPMC11527082biostudies-literatureGenetic diversity in the Plasmodium falciparum next-generation blood stage vaccine candidate antigen PfCyRPA in Senegal.PMC11527082R01 AI168238K01 TW010496Sheng ZGuo YMoore AJPatel SDLi RShapiro LPouye MNMbengue ADiallo FSene SDLaqqa SNdiaye JLAMangou KCisse AGuerra NThiam LGSadio BDBei AKBa AfalseGenetic diversity in the Plasmodium falciparum next-generation blood stage vaccine candidate antigen PfCyRPA in Senegal.The <i>Plasmodium falciparum</i> cysteine-rich protective antigen (PfCyRPA) is a promising target as a next-generation blood-stage malaria vaccine and together with PCRCR complex members, the reticulocyte binding-like homologous protein 5 (PfRh5) and the Rh5-interacting protein (PfRipr), are currently being evaluated in clinical trials. PfCyRPA is essential for merozoite invasion and appears to be highly conserved within the <i>P. falciparum</i> parasite populations. Here, we used a targeted deep amplicon next-generation sequencing approach to assess the breadth of PfCyRPA genetic diversity in 95 <i>P. falciparum</i> clinical isolates from Kédougou, an area with a high seasonal malaria transmission in Senegal. Our data show the dominant prevalence of PfCyRPA wild type reference allele, whi2024-01-01T00:00:00Z2024 Oct2026-07-02T03:15:32.045Z2025-04-06T10:11:39.417ZS-EPMC115270823948425710.1101/2024.10.13.24305808