biostudies-literatureLi TMinistry of Science and Technology of ChinaChinese Academy of SciencesNational Science and Technology Council TaiwanMinistry of Education (MOE) in TaiwanNational Science Foundation of ChinaRP87258https://www.ebi.ac.uk/biostudies/studies/S-EPMC11527428biostudies-literatureUnknown12Traumatic brain injury (TBI) caused by external mechanical forces is a major health burden worldwide, but the underlying mechanism in glia remains largely unclear. We report herein that <i>Drosophila</i> adults exhibit a defective blood-brain barrier, elevated innate immune responses, and astrocyte swelling upon consecutive strikes with a high-impact trauma device. RNA sequencing (RNA-seq) analysis of these astrocytes revealed upregulated expression of genes encoding PDGF and VEGF receptor-related (Pvr, a receptor tyrosine kinase), adaptor protein complex 1 (AP-1<i>,</i> a transcription factor complex of the c-Jun N-terminal kinase pathway) composed of Jun-related antigen (Jra) and kayak (kay), and matrix metalloproteinase 1 (Mmp1) following TBI. Interestingly, Pvr is both required and sufeLifeA Pvr-AP-1-Mmp1 signaling pathway is activated in astrocytes upon traumatic brain injury.PMC1152742831921002321709622021ZD0203900113-2628-B-A49-0072019YFA070710031861143031Higher Education Sprout Project31830036Strategic Priority Research Program B XDBS1020100Li THo MSShi WZhang YQfalseA Pvr-AP-1-Mmp1 signaling pathway is activated in astrocytes upon traumatic brain injury.Traumatic brain injury (TBI) caused by external mechanical forces is a major health burden worldwide, but the underlying mechanism in glia remains largely unclear. We report herein that <i>Drosophila</i> adults exhibit a defective blood-brain barrier, elevated innate immune responses, and astrocyte swelling upon consecutive strikes with a high-impact trauma device. RNA sequencing (RNA-seq) analysis of these astrocytes revealed upregulated expression of genes encoding PDGF and VEGF receptor-related (Pvr, a receptor tyrosine kinase), adaptor protein complex 1 (AP-1<i>,</i> a transcription factor complex of the c-Jun N-terminal kinase pathway) composed of Jun-related antigen (Jra) and kayak (kay), and matrix metalloproteinase 1 (Mmp1) following TBI. Interestingly, Pvr is both required and suf2024-01-01T00:00:00Z2024 Oct2025-04-05T09:12:34.313Z2025-04-05T09:12:34.313ZS-EPMC115274283948070410.7554/eLife.87258