biostudies-literatureMichels EHAZonMw1470383https://www.ebi.ac.uk/biostudies/studies/S-EPMC11527607biostudies-literatureUnknown15<h4>Introduction</h4>Neutrophils play a dichotomous role in community-acquired pneumonia (CAP), providing protection and potentially causing damage. Existing research on neutrophil function in CAP relies on animal studies, leaving a gap in patient-centered investigations.<h4>Methods</h4>We used mass spectrometry to characterize the neutrophil proteome of moderately ill CAP patients at general ward admission and related the proteome to controls and clinical outcomes.<h4>Results</h4>We prospectively included 57 CAP patients and 26 controls and quantified 3482 proteins in neutrophil lysates and 386 proteins in concurrently collected plasma. The extensively studied granule-related proteins in animal models did not drive the neutrophil proteome changes associated with human CAP. Proteome alteraFrontiers in immunologyProteomic profiling of neutrophils and plasma in community-acquired pneumonia reveals crucial proteins in diverse biological pathways linked to clinical outcome.PMC1152760710430142110001de Vos AFHoogendijk AJWiersinga WJvan der Poll TMichels EHAChouchane ODouma RAFaber DRde Brabander JSmit ERvan den Biggelaar MfalseProteomic profiling of neutrophils and plasma in community-acquired pneumonia reveals crucial proteins in diverse biological pathways linked to clinical outcome.<h4>Introduction</h4>Neutrophils play a dichotomous role in community-acquired pneumonia (CAP), providing protection and potentially causing damage. Existing research on neutrophil function in CAP relies on animal studies, leaving a gap in patient-centered investigations.<h4>Methods</h4>We used mass spectrometry to characterize the neutrophil proteome of moderately ill CAP patients at general ward admission and related the proteome to controls and clinical outcomes.<h4>Results</h4>We prospectively included 57 CAP patients and 26 controls and quantified 3482 proteins in neutrophil lysates and 386 proteins in concurrently collected plasma. The extensively studied granule-related proteins in animal models did not drive the neutrophil proteome changes associated with human CAP. Proteome altera2024-01-01T00:00:00Z20242026-07-16T09:28:34.815Z2025-04-21T21:41:19.727ZS-EPMC115276073949375510.3389/fimmu.2024.1470383