biostudies-literatureChiba ATakeda Science FoundationNishinomiya Basic Research FundJapan Society for the Promotion of Science516-527https://www.ebi.ac.uk/biostudies/studies/S-EPMC11529416biostudies-literatureUnknown44(11)Parkinson's disease (PD) is an age-related progressive neurodegenerative disease. Previously, we identified midnolin (<i>MIDN</i>) as a genetic risk factor for PD. Although <i>MIDN</i> copy number loss increases the risk of PD, the molecular function of MIDN remains unclear. To investigate the role of MIDN in PD, we established monoclonal <i>Midn</i> knockout (KO) PC12 cell models. <i>Midn</i> KO inhibited neurite outgrowth and neurofilament light chain (<i>Nefl</i>) gene expression. Although MIDN is mainly localized in the nucleus, it does not encode DNA-binding domains. We therefore hypothesized that MIDN might bind to certain transcription factors and regulate gene expression. Of the candidate transcription factors, we focused on early growth response 1 (EGR1) because it is required forMolecular and cellular biologyMidnolin, a Genetic Risk Factor for Parkinson's Disease, Promotes Neurite Outgrowth Accompanied by Early Growth Response 1 Activation in PC12 Cells.PMC1152941621K06573Nagashima MNakamura KObara YIshii KHosoi TChiba ANakagawa TKato COsaki TNorota IfalseMidnolin, a Genetic Risk Factor for Parkinson's Disease, Promotes Neurite Outgrowth Accompanied by Early Growth Response 1 Activation in PC12 Cells.Parkinson's disease (PD) is an age-related progressive neurodegenerative disease. Previously, we identified midnolin (<i>MIDN</i>) as a genetic risk factor for PD. Although <i>MIDN</i> copy number loss increases the risk of PD, the molecular function of MIDN remains unclear. To investigate the role of MIDN in PD, we established monoclonal <i>Midn</i> knockout (KO) PC12 cell models. <i>Midn</i> KO inhibited neurite outgrowth and neurofilament light chain (<i>Nefl</i>) gene expression. Although MIDN is mainly localized in the nucleus, it does not encode DNA-binding domains. We therefore hypothesized that MIDN might bind to certain transcription factors and regulate gene expression. Of the candidate transcription factors, we focused on early growth response 1 (EGR1) because it is required for2024-01-01T00:00:00Z20242025-04-18T12:59:25.968Z2025-04-04T01:58:21.54ZS-EPMC115294163926436110.1080/10985549.2024.2399358