biostudies-literatureZhou XWFujian forestry science and technology projectFoundation for Innovative Research Groups of the National Natural Science Foundation of Chinathe project of scientific research foundation in Yulin Normal Universitythe Key Program of Guangxi Nature Science Foundationthe scientific research foundation project of Fujian University of Technologythe project of improving the research capabilities of young and middle-aged teachers in Guangxi Zhuang Autonomous Region1037https://www.ebi.ac.uk/biostudies/studies/S-EPMC11529430biostudies-literatureUnknown24(1)<h4>Background</h4>Camellia nitidissima Chi is a popular ornamental plant because of its golden flowers, which contain flavonoids and carotenoids. To understand the regulatory mechanism of golden color formation, the metabolites of C. nitidissima petals at five different developmental stages were detected, a proteome map of petals was first constructed via tandem mass tag (TMT) analysis, and the accuracy of the sequencing data was validated via parallel reaction monitoring (PRM).<h4>Results</h4>Nineteen color components were detected, and most of these components were carotenoids that gradually accumulated, while some metabolites were flavonoids that were gradually depleted. A total of 97,647 spectra were obtained, and 6,789 quantifiable proteins were identified. Then, 1,319 differentiallyBMC plant biologyProteomic analysis identified proteins that are differentially expressed in the flavonoid and carotenoid biosynthetic pathways of Camellia Nitidissima flowers.PMC11529430G2019ZK13, G2019ZK352022KY0577GY-Z22041318602282018GXNSFDA281007ZMGG-0702Ye XXZhou XWHu HBXie YQLiu HXLiu ZJXu QYYan SHYe BJLi BYao XfalseProteomic analysis identified proteins that are differentially expressed in the flavonoid and carotenoid biosynthetic pathways of Camellia Nitidissima flowers.<h4>Background</h4>Camellia nitidissima Chi is a popular ornamental plant because of its golden flowers, which contain flavonoids and carotenoids. To understand the regulatory mechanism of golden color formation, the metabolites of C. nitidissima petals at five different developmental stages were detected, a proteome map of petals was first constructed via tandem mass tag (TMT) analysis, and the accuracy of the sequencing data was validated via parallel reaction monitoring (PRM).<h4>Results</h4>Nineteen color components were detected, and most of these components were carotenoids that gradually accumulated, while some metabolites were flavonoids that were gradually depleted. A total of 97,647 spectra were obtained, and 6,789 quantifiable proteins were identified. Then, 1,319 differentially2024-01-01T00:00:00Z2024 Nov2026-07-15T13:09:50.638Z2025-04-06T03:13:47.724ZS-EPMC115294303948257410.1186/s12870-024-05737-7