{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Messaoudi S"],"funding":["Natural Sciences and Engineering Research Council of Canada","Cancer Research Society","Fonds de Recherche du Québec - Santé","Prostate Cancer Canada","Canadian Institutes of Health Research"],"pagination":["29824-29835"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11529601"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["146(43)"],"pubmed_abstract":["Bispecific antibodies (bsAbs) are emerging immune-therapeutics, and many formats exist that differ considerably in structure. However, little systematic data exist about how the spatial organization of their components influences activity, requiring innovative approaches combining empirical and quantitative frameworks. This study presents a modular DNA nanotechnology platform to generate numerous bsAbs with surrogate geometries that span the structural features of the BiTE, IgG-like, and IgG-conjugate platforms to screen for T-cell engagement. Results highlight interesting structure-activity relationships regarding bsAb potency and selectivity and raise questions regarding the molecular phenomena underlying activity. To elucidate some effects, the platform was paired with a simple mathemat"],"journal":["Journal of the American Chemical Society"],"pubmed_title":["Rapid Systematic Screening of Bispecific Antibody Surrogate Geometries for T-Cell Engagement Using DNA Nanotechnology."],"pmcid":["PMC11529601"],"funding_grant_id":["438050","400399","RGPIN- 201504254"],"pubmed_authors":["Marple A","Pelletier M","Durocher Y","Gauthier MA","Wylie RG","Baniahmad SF","Messaoudi S","Greschner AA","Wai K","Craig M"],"additional_accession":[]},"is_claimable":false,"name":"Rapid Systematic Screening of Bispecific Antibody Surrogate Geometries for T-Cell Engagement Using DNA Nanotechnology.","description":"Bispecific antibodies (bsAbs) are emerging immune-therapeutics, and many formats exist that differ considerably in structure. However, little systematic data exist about how the spatial organization of their components influences activity, requiring innovative approaches combining empirical and quantitative frameworks. This study presents a modular DNA nanotechnology platform to generate numerous bsAbs with surrogate geometries that span the structural features of the BiTE, IgG-like, and IgG-conjugate platforms to screen for T-cell engagement. Results highlight interesting structure-activity relationships regarding bsAb potency and selectivity and raise questions regarding the molecular phenomena underlying activity. To elucidate some effects, the platform was paired with a simple mathemat","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2026-06-04T11:46:39.562Z","creation":"2026-05-08T03:10:59Z"},"accession":"S-EPMC11529601","cross_references":{"pubmed":["39412838"],"doi":["10.1021/jacs.4c11648"]}}