biostudies-literatureNguyen LPNOVO Nordisk FoundationNHLBINHLBI NIH HHSKnut and Alice Wallenberg FoundationSwedish Cancer SocietyLeducq FoundationSwedish Research CouncilNovo Nordisk Fondene184940https://www.ebi.ac.uk/biostudies/studies/S-EPMC11529983biostudies-literatureUnknown9(20)Lipoprotein lipase (LPL) and multiple regulators of LPL activity (e.g., APOC2 and ANGPTL4) are present in all vertebrates, but GPIHBP1-the endothelial cell (EC) protein that captures LPL within the subendothelial spaces and transports it to its site of action in the capillary lumen-is present in mammals but in not chickens or other lower vertebrates. In mammals, GPIHBP1 deficiency causes severe hypertriglyceridemia, but chickens maintain low triglyceride levels despite the absence of GPIHBP1. To understand intravascular lipolysis in lower vertebrates, we examined LPL expression in mouse and chicken hearts. In both species, LPL was abundant on capillaries, but the distribution of Lpl transcripts was strikingly different. In mouse hearts, Lpl transcripts were extremely abundant in cardiomyocJCI insightDistinct strategies for intravascular triglyceride metabolism in hearts of mammals and lower vertebrate species.PMC11529983R01 HL0872282020.00571507352015–00550HL171737,HL146358,HL087228,HL139725P01 HL146358NNF20OC00634442023-02655R35 HL13972512CVD04,22CVD01,23CVD02R01 HL171737Birrane GYu RGPloug MFong LGScheithauer JArnold HGoodwin JLJung HMae MAKoltowska KHe LKim JRYang YNguyen LPXie KPresnell AMTran APWeston TAKim PHYoung SGSong WTu YBeigneux APBetsholtz CfalseDistinct strategies for intravascular triglyceride metabolism in hearts of mammals and lower vertebrate species.Lipoprotein lipase (LPL) and multiple regulators of LPL activity (e.g., APOC2 and ANGPTL4) are present in all vertebrates, but GPIHBP1-the endothelial cell (EC) protein that captures LPL within the subendothelial spaces and transports it to its site of action in the capillary lumen-is present in mammals but in not chickens or other lower vertebrates. In mammals, GPIHBP1 deficiency causes severe hypertriglyceridemia, but chickens maintain low triglyceride levels despite the absence of GPIHBP1. To understand intravascular lipolysis in lower vertebrates, we examined LPL expression in mouse and chicken hearts. In both species, LPL was abundant on capillaries, but the distribution of Lpl transcripts was strikingly different. In mouse hearts, Lpl transcripts were extremely abundant in cardiomyoc2024-01-01T00:00:00Z2024 Sep2026-05-04T06:39:29.835Z2025-04-06T09:32:19.025ZS-EPMC115299833943566110.1172/jci.insight.184940