{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Muthaliff NVMA"],"funding":["Agency for Science, Technology and Research"],"pagination":["500"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11530489"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["108(1)"],"pubmed_abstract":["9-Carbon aldehydes such as (2E)-nonenal, (3Z)-nonenal, and (2E,6Z)-nonadienal are important melon and cucumber fragrance compounds. Currently, these molecules are produced either synthetically, which faces consumer aversion, or through biotransformation using plant-extracted enzymes, which is costly and inefficient. In this study, we constructed a Saccharomyces cerevisiae platform for the whole cell biotransformation of polyunsaturated fatty acids (PUFAs) to 9-carbon aldehydes. Heterologous expression of lipoxygenase (LOX) from Nicotiana benthamiana and hydroperoxide lyase (HPL) from Cucumis melo (melon) in S. cerevisiae enabled the production of (2E)-nonenal from readily available polyunsaturated fatty acid substrates. A 5.5-fold increase in (2E)-nonenal titer was then achieved utilizing "],"journal":["Applied microbiology and biotechnology"],"pubmed_title":["Saccharomyces cerevisiae whole cell biotransformation for the production of aldehyde flavors and fragrances."],"pmcid":["PMC11530489"],"funding_grant_id":["H20H6a0028"],"pubmed_authors":["Ng YZ","Muthaliff NVMA","Ang EL","Guo WM"],"additional_accession":[]},"is_claimable":false,"name":"Saccharomyces cerevisiae whole cell biotransformation for the production of aldehyde flavors and fragrances.","description":"9-Carbon aldehydes such as (2E)-nonenal, (3Z)-nonenal, and (2E,6Z)-nonadienal are important melon and cucumber fragrance compounds. Currently, these molecules are produced either synthetically, which faces consumer aversion, or through biotransformation using plant-extracted enzymes, which is costly and inefficient. In this study, we constructed a Saccharomyces cerevisiae platform for the whole cell biotransformation of polyunsaturated fatty acids (PUFAs) to 9-carbon aldehydes. Heterologous expression of lipoxygenase (LOX) from Nicotiana benthamiana and hydroperoxide lyase (HPL) from Cucumis melo (melon) in S. cerevisiae enabled the production of (2E)-nonenal from readily available polyunsaturated fatty acid substrates. A 5.5-fold increase in (2E)-nonenal titer was then achieved utilizing ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-06-02T04:08:40.084Z","creation":"2025-04-21T21:44:45.638Z"},"accession":"S-EPMC11530489","cross_references":{"pubmed":["39485521"],"doi":["10.1007/s00253-024-13335-8"]}}