{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["MacDonald AI"],"funding":["Biotechnology and Biological Sciences Research Council","National Science Foundation"],"pagination":["26280"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11530663"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Serine integrases are phage- (or mobile element-) encoded enzymes that catalyse site-specific recombination reactions between a short DNA sequence on the phage genome (attP) and a corresponding host genome sequence (attB), thereby integrating the phage DNA into the host genome. Each integrase has its unique pair of attP and attB sites, a feature that allows them to be used as orthogonal tools for genome modification applications. In the presence of a second protein, the Recombination Directionality Factor (RDF), integrase catalyses the reverse excisive reaction, generating new recombination sites, attR and attL. In addition to promoting attR x attL reaction, the RDF inhibits attP x attB recombination. This feature makes the directionality of integrase reactions programmable, allowing them "],"journal":["Scientific reports"],"pubmed_title":["Variable orthogonality of serine integrase interactions within the ϕC31 family."],"pmcid":["PMC11530663"],"funding_grant_id":["BB/003356/1","BB/X012085/1","2223480"],"pubmed_authors":["MacDonald AI","Rice PA","Olorunniji FJ","Baksh A","Holland A","Shin H","Stark WM"],"additional_accession":[]},"is_claimable":false,"name":"Variable orthogonality of serine integrase interactions within the ϕC31 family.","description":"Serine integrases are phage- (or mobile element-) encoded enzymes that catalyse site-specific recombination reactions between a short DNA sequence on the phage genome (attP) and a corresponding host genome sequence (attB), thereby integrating the phage DNA into the host genome. Each integrase has its unique pair of attP and attB sites, a feature that allows them to be used as orthogonal tools for genome modification applications. In the presence of a second protein, the Recombination Directionality Factor (RDF), integrase catalyses the reverse excisive reaction, generating new recombination sites, attR and attL. In addition to promoting attR x attL reaction, the RDF inhibits attP x attB recombination. This feature makes the directionality of integrase reactions programmable, allowing them ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-06-02T04:01:35.488Z","creation":"2025-04-06T23:17:45.403Z"},"accession":"S-EPMC11530663","cross_references":{"pubmed":["39487291"],"doi":["10.1038/s41598-024-77570-9"]}}