{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wu J"],"funding":["the National Key R&D Program of China","National Natural Science Foundation of China","Ministry of Agriculture and Rural Affairs of the People's Republic of China","National Natural Science Foundation of China (National Science Foundation of China)"],"pagination":["9438"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11530683"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["Mitochondria serve as the cellular powerhouse, and their distinct DNA makes them a prospective target for gene editing to treat genetic disorders. However, the impact of genome editing on mitochondrial DNA (mtDNA) stability remains a mystery. Our study reveals previously unknown risks of genome editing that both nuclear and mitochondrial editing cause discernible transfer of mitochondrial DNA segments into the nuclear genome in various cell types including human cell lines, primary T cells, and mouse embryos. Furthermore, drug-induced mitochondrial stresses and mtDNA breaks exacerbate this transfer of mtDNA into the nuclear genome. Notably, we observe that mitochondrial editors, including mitoTALEN and recently developed base editor DdCBE, can also enhance crosstalk between mtDNA and the n"],"journal":["Nature communications"],"pubmed_title":["Transfer of mitochondrial DNA into the nuclear genome during induced DNA breaks."],"pmcid":["PMC11530683"],"funding_grant_id":["92254305","32225025","32122108"],"pubmed_authors":["Wu J","Yuan S","Li J","Liu M","Zhangding Z","Xin C","Liu X","Liu Y","Hu J","Ou L","Tian Y","Gan T","Yin J"],"additional_accession":[]},"is_claimable":false,"name":"Transfer of mitochondrial DNA into the nuclear genome during induced DNA breaks.","description":"Mitochondria serve as the cellular powerhouse, and their distinct DNA makes them a prospective target for gene editing to treat genetic disorders. However, the impact of genome editing on mitochondrial DNA (mtDNA) stability remains a mystery. Our study reveals previously unknown risks of genome editing that both nuclear and mitochondrial editing cause discernible transfer of mitochondrial DNA segments into the nuclear genome in various cell types including human cell lines, primary T cells, and mouse embryos. Furthermore, drug-induced mitochondrial stresses and mtDNA breaks exacerbate this transfer of mtDNA into the nuclear genome. Notably, we observe that mitochondrial editors, including mitoTALEN and recently developed base editor DdCBE, can also enhance crosstalk between mtDNA and the n","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-07-16T07:37:08.254Z","creation":"2025-04-19T18:12:49.125Z"},"accession":"S-EPMC11530683","cross_references":{"pubmed":["39487167"],"doi":["10.1038/s41467-024-53806-0"]}}