biostudies-literaturePapouskova AUniversity of Veterinary Sciences2901-2905https://www.ebi.ac.uk/biostudies/studies/S-EPMC11531815biostudies-literatureUnknown79(11)<h4>Objectives</h4>We performed a retrospective analysis of MRSA isolates collected at the university equine clinic including clinical isolates from 2008 to 2021 and screening environmental, equine and personnel isolates from 2016.<h4>Methods</h4>Screening and clinical samples were cultured on Brilliance MRSA 2 and Columbia agar (Oxoid), respectively, with enrichment for environmental samples. Antimicrobial susceptibility was assessed by disc diffusion. All the isolates were characterized by spa typing. Eighteen selected isolates were subjected to WGS with subsequent wgMLST clonal analysis.<h4>Results</h4>Among 75 MRSA isolates, five spa types were identified, the majority (n = 67; 89.33%) was t011. All isolates were resistant to cefoxitin and ampicillin and carried the mecA gene. In additThe Journal of antimicrobial chemotherapyThe circulation of methicillin-resistant Staphylococcus aureus between humans, horses and the environment at the equine clinic.PMC11531815Brno No. 2022 ITA 31Krutova MDrabkova ZPapouskova ABrajerova MCizek ATkadlec JfalseThe circulation of methicillin-resistant Staphylococcus aureus between humans, horses and the environment at the equine clinic.<h4>Objectives</h4>We performed a retrospective analysis of MRSA isolates collected at the university equine clinic including clinical isolates from 2008 to 2021 and screening environmental, equine and personnel isolates from 2016.<h4>Methods</h4>Screening and clinical samples were cultured on Brilliance MRSA 2 and Columbia agar (Oxoid), respectively, with enrichment for environmental samples. Antimicrobial susceptibility was assessed by disc diffusion. All the isolates were characterized by spa typing. Eighteen selected isolates were subjected to WGS with subsequent wgMLST clonal analysis.<h4>Results</h4>Among 75 MRSA isolates, five spa types were identified, the majority (n = 67; 89.33%) was t011. All isolates were resistant to cefoxitin and ampicillin and carried the mecA gene. In addit2024-01-01T00:00:00Z2024 Nov2026-07-09T11:23:53.326Z2025-04-04T22:48:13.669ZS-EPMC115318153921216710.1093/jac/dkae303