biostudies-literatureYoshida HJapan Society for the Promotion of Science1028https://www.ebi.ac.uk/biostudies/studies/S-EPMC11533360biostudies-literatureUnknown25(1)<h4>Background</h4>Streptococcus canis is a commensal bacterium in companion animals. This microorganism can infect humans who have been in deep contact with or bitten by pet dogs, suggesting that the skin/soft tissue is one of infection entry sites. To understand pathological process in human cells, we aimed to determine S. canis transcriptomic changes in invasive environments of human keratinocytes.<h4>Methods</h4>We selected one isolate from candidates with whole-genome sequences, based on re-obtained cell invasion ability (CIA) data into human keratinocytes along with bacterial cytotoxicity. RNA-sequencing was conducted for the samples at baselines and 2 h/5 hr post-inoculation using NovaSeq 6000. Global/differential gene expression analyses [principal component analysis (PCA)/k-means BMC genomicsStreptococcus canis transcriptomic modifications in host cell entry environments of human keratinocytes.PMC1153336021K08513Takahashi TTsuyuki YGoto MYoshida HKim JSfalseStreptococcus canis transcriptomic modifications in host cell entry environments of human keratinocytes.<h4>Background</h4>Streptococcus canis is a commensal bacterium in companion animals. This microorganism can infect humans who have been in deep contact with or bitten by pet dogs, suggesting that the skin/soft tissue is one of infection entry sites. To understand pathological process in human cells, we aimed to determine S. canis transcriptomic changes in invasive environments of human keratinocytes.<h4>Methods</h4>We selected one isolate from candidates with whole-genome sequences, based on re-obtained cell invasion ability (CIA) data into human keratinocytes along with bacterial cytotoxicity. RNA-sequencing was conducted for the samples at baselines and 2 h/5 hr post-inoculation using NovaSeq 6000. Global/differential gene expression analyses [principal component analysis (PCA)/k-means 2024-01-01T00:00:00Z2024 Nov2026-06-02T23:01:06.348Z2025-04-04T22:47:22.282ZS-EPMC115333603949705110.1186/s12864-024-10974-z