{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Matsui A"],"funding":["Jennifer and Bernard LaCroute","National Institute of Neurological Disorders & Stroke","National Institute of Neurological Disorders & Stroke","Howard Hughes Medical Institute","National Cancer Institute","NCI NIH HHS","NINDS NIH HHS"],"pagination":["RP98458"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11534335"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["Chemical synapses are the major sites of communication between neurons in the nervous system and mediate either excitatory or inhibitory signaling. At excitatory synapses, glutamate is the primary neurotransmitter and upon release from presynaptic vesicles, is detected by postsynaptic glutamate receptors, which include ionotropic AMPA and NMDA receptors. Here, we have developed methods to identify glutamatergic synapses in brain tissue slices, label AMPA receptors with small gold nanoparticles (AuNPs), and prepare lamella for cryo-electron tomography studies. The targeted imaging of glutamatergic synapses in the lamella is facilitated by fluorescent pre- and postsynaptic signatures, and the subsequent tomograms allow for the identification of key features of chemical synapses, including sy"],"journal":["eLife"],"pubmed_title":["Cryo-electron tomographic investigation of native hippocampal glutamatergic synapses."],"pmcid":["PMC11534335"],"funding_grant_id":["R37 NS038631","NS038631","R01 NS038631","CA253730","F99 CA253730","K00 CA253730"],"pubmed_authors":["Gouaux E","Jean N","Qin M","Shiozaki M","Elferich J","Zhong H","Spangler C","Matsui A","Yu Z","Zhao X"],"additional_accession":[]},"is_claimable":false,"name":"Cryo-electron tomographic investigation of native hippocampal glutamatergic synapses.","description":"Chemical synapses are the major sites of communication between neurons in the nervous system and mediate either excitatory or inhibitory signaling. At excitatory synapses, glutamate is the primary neurotransmitter and upon release from presynaptic vesicles, is detected by postsynaptic glutamate receptors, which include ionotropic AMPA and NMDA receptors. Here, we have developed methods to identify glutamatergic synapses in brain tissue slices, label AMPA receptors with small gold nanoparticles (AuNPs), and prepare lamella for cryo-electron tomography studies. The targeted imaging of glutamatergic synapses in the lamella is facilitated by fluorescent pre- and postsynaptic signatures, and the subsequent tomograms allow for the identification of key features of chemical synapses, including sy","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-06-03T07:21:37.185Z","creation":"2025-04-06T09:35:14.849Z"},"accession":"S-EPMC11534335","cross_references":{"pubmed":["39495821"],"doi":["10.7554/eLife.98458"]}}