{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Brunetta HS"],"funding":["Deutsche Forschungsgemeinschaft (DFG)","Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)","European Research Council","Conselho Nacional de Desenvolvimento Científico e Tecnológico","Deutsche Forschungsgemeinschaft","Deutsches Zentrum für Herz-Kreislaufforschung","Coordenação de Aperfeiçoamento de Pessoal de Nível Superior","Fundação de Amparo à Pesquisa do Estado de São Paulo","Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)","Deutsches Zentrum für Herz-Kreislaufforschung (DZHK)","Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)"],"pagination":["4870-4891"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11535227"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["43(21)"],"pubmed_abstract":["While mechanisms controlling uncoupling protein-1 (UCP1) in thermogenic adipocytes play a pivotal role in non-shivering thermogenesis, it remains unclear whether F<sub>1</sub>Fo-ATP synthase function is also regulated in brown adipose tissue (BAT). Here, we show that inhibitory factor 1 (IF1, encoded by Atp5if1), an inhibitor of ATP synthase hydrolytic activity, is a critical negative regulator of brown adipocyte energy metabolism. In vivo, IF1 levels are diminished in BAT of cold-adapted mice compared to controls. Additionally, the capacity of ATP synthase to generate mitochondrial membrane potential (MMP) through ATP hydrolysis (the so-called \"reverse mode\" of ATP synthase) is increased in brown fat. In cultured brown adipocytes, IF1 overexpression results in an inability of mitochondria"],"journal":["The EMBO journal"],"pubmed_title":["IF1 is a cold-regulated switch of ATP synthase hydrolytic activity to support thermogenesis in brown fat."],"pmcid":["PMC11535227"],"funding_grant_id":["310287/2018-9","852742","2022/00358-1","88881.143924/2017-01","BA4925/2-1","81X3600212"],"pubmed_authors":["Valdivieso-Rivera F","Francisco A","Furino VO","Keipert S","Brunetta HS","Castilho RF","Jastroch M","Bercot M","Guerra J","Martinez LO","de Campos Zani SC","Mori MA","Moraes-Vieira PM","Bartelt A","Jung AS","Sponton CH"],"additional_accession":[]},"is_claimable":false,"name":"IF1 is a cold-regulated switch of ATP synthase hydrolytic activity to support thermogenesis in brown fat.","description":"While mechanisms controlling uncoupling protein-1 (UCP1) in thermogenic adipocytes play a pivotal role in non-shivering thermogenesis, it remains unclear whether F<sub>1</sub>Fo-ATP synthase function is also regulated in brown adipose tissue (BAT). Here, we show that inhibitory factor 1 (IF1, encoded by Atp5if1), an inhibitor of ATP synthase hydrolytic activity, is a critical negative regulator of brown adipocyte energy metabolism. In vivo, IF1 levels are diminished in BAT of cold-adapted mice compared to controls. Additionally, the capacity of ATP synthase to generate mitochondrial membrane potential (MMP) through ATP hydrolysis (the so-called \"reverse mode\" of ATP synthase) is increased in brown fat. In cultured brown adipocytes, IF1 overexpression results in an inability of mitochondria","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-07-16T13:20:06.325Z","creation":"2025-04-20T03:55:41.315Z"},"accession":"S-EPMC11535227","cross_references":{"pubmed":["39284909"],"doi":["10.1038/s44318-024-00215-0"]}}