{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14(1)"],"submitter":["Aquinas N"],"funding":["Manipal Academy of Higher Education, Manipal"],"pubmed_abstract":["In this study, the upstream and downstream production processes of curdlan from Priestia megaterium were optimized to enhance its yield. Additionally, a novel extraction method was developed for curdlan recovery. Optimization studies were conducted using Central composite design (CCD). Curdlan yield improved from 0.15 g/L (unoptimized) to 0.46 g/L (3-fold increase) when fermentation was carried out in CCD-optimized media of (w/v) sucrose 20%, urea 0.1%, KH<sub>2</sub>PO<sub>4</sub> 0.02%, agitation speed 250 rpm. To further enhance curdlan yield during extraction, ultrasonication was incorporated as a novel step into the conventional method of acid/alkali-assisted curdlan recovery. A two-step optimization was chosen for extraction, namely, one-factor-at-a-time (OFAT) and CCD, wherein the o"],"journal":["Scientific reports"],"pagination":["26709"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11535322"],"repository":["biostudies-literature"],"pubmed_title":["Optimization of curdlan production and ultrasound assisted extraction processes from Priestia megaterium."],"pmcid":["PMC11535322"],"pubmed_authors":["Ramananda BM","Aquinas N","Selvaraj S"],"additional_accession":[]},"is_claimable":false,"name":"Optimization of curdlan production and ultrasound assisted extraction processes from Priestia megaterium.","description":"In this study, the upstream and downstream production processes of curdlan from Priestia megaterium were optimized to enhance its yield. Additionally, a novel extraction method was developed for curdlan recovery. Optimization studies were conducted using Central composite design (CCD). Curdlan yield improved from 0.15 g/L (unoptimized) to 0.46 g/L (3-fold increase) when fermentation was carried out in CCD-optimized media of (w/v) sucrose 20%, urea 0.1%, KH<sub>2</sub>PO<sub>4</sub> 0.02%, agitation speed 250 rpm. To further enhance curdlan yield during extraction, ultrasonication was incorporated as a novel step into the conventional method of acid/alkali-assisted curdlan recovery. A two-step optimization was chosen for extraction, namely, one-factor-at-a-time (OFAT) and CCD, wherein the o","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2025-04-20T03:54:56.008Z","creation":"2025-04-20T03:54:56.008Z"},"accession":"S-EPMC11535322","cross_references":{"pubmed":["39496699"],"doi":["10.1038/s41598-024-77880-y"]}}