{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Drosu N"],"funding":["National Multiple Sclerosis Society (NMSS)","National Multiple Sclerosis Society"],"pagination":["e2416097121"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11536159"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["121(44)"],"pubmed_abstract":["Both genetic and environmental factors contribute to multiple sclerosis (MS) risk. Infection with the Epstein-Barr virus (EBV) is the strongest environmental risk factor, and HLA-DR15 is the strongest genetic risk factor for MS. We employed computational methods and in vitro assays for CD4 T cell activation to investigate the DR15-restricted response to EBV. Using a machine learning-based HLA ligand predictor, the EBV glycoprotein B (gB) was predicted to be enriched in epitopes restricted to presentation by DRB1*15:01. In DR15-positive individuals, two epitopes comprised the major CD4 T cell response to gB. Surprisingly, the expression of recombinant gB in a DR15-homozygous B cell line or primary autologous B cells elicited a CD4 T cell response, indicating that intracellular gB was loaded"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["CD4 T cells restricted to DRB1*15:01 recognize two Epstein-Barr virus glycoproteins capable of intracellular antigen presentation."],"pmcid":["PMC11536159"],"funding_grant_id":["2022A011428"],"pubmed_authors":["Nishiyama S","Anderson M","Drosu N","Cabot J","Bobrowski-Khoury N","Bilodeau PA","Mikami T","Housman D","Levy M"],"additional_accession":[]},"is_claimable":false,"name":"CD4 T cells restricted to DRB1*15:01 recognize two Epstein-Barr virus glycoproteins capable of intracellular antigen presentation.","description":"Both genetic and environmental factors contribute to multiple sclerosis (MS) risk. Infection with the Epstein-Barr virus (EBV) is the strongest environmental risk factor, and HLA-DR15 is the strongest genetic risk factor for MS. We employed computational methods and in vitro assays for CD4 T cell activation to investigate the DR15-restricted response to EBV. Using a machine learning-based HLA ligand predictor, the EBV glycoprotein B (gB) was predicted to be enriched in epitopes restricted to presentation by DRB1*15:01. In DR15-positive individuals, two epitopes comprised the major CD4 T cell response to gB. Surprisingly, the expression of recombinant gB in a DR15-homozygous B cell line or primary autologous B cells elicited a CD4 T cell response, indicating that intracellular gB was loaded","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2026-06-01T06:18:09.23Z","creation":"2026-04-08T09:46:44.362Z"},"accession":"S-EPMC11536159","cross_references":{"pubmed":["39432795"],"doi":["10.1073/pnas.2416097121"]}}