biostudies-literatureChen ZGuangzhou Major Clinical Technology ProjectGuangzhou Municipal Science and Technology Plan ProjectGuangdong Key Laboratory of Plant Adaptation and Molecular DesignNational Natural Science Foundation of China2420612https://www.ebi.ac.uk/biostudies/studies/S-EPMC11536693biostudies-literatureUnknown16(1)<h4>Background</h4>Probiotics serve as a novel preventive or therapeutic approach for dental caries owing to their ability to reverse dysbiosis and restore a healthy microbiota. Here, we identified <i>Burkholderia ambifaria</i> AFS098024 as a probiotic candidate isolated from plants.<h4>Methods</h4>The safety of <i>B. ambifaria</i> was evaluated by hemolytic activity, D-lactic acid production and antibiotic susceptibility. <i>In vitro</i> biofilm model derived from the saliva of caries-free and caries-active donors and <i>in vivo</i> rat caries model were used to assess the efficacy of <i>B. ambifaria</i> in caries prevention and treatment.<h4>Results</h4><i>B. ambifaria</i> was safe as a probiotic candidate and it could integrate with <i>in vitro</i> biofilm model. It significantly reduceJournal of oral microbiologyEvaluation of a <i>Burkholderia ambifaria</i> strain from plants as a novel promising probiotic in dental caries management.PMC11536693No. 202201011112No. 2023P-ZD07No. 82201041No. 2022B1212010013-6Wu LChen ZLu YCai YWei XXu ZfalseEvaluation of a <i>Burkholderia ambifaria</i> strain from plants as a novel promising probiotic in dental caries management.<h4>Background</h4>Probiotics serve as a novel preventive or therapeutic approach for dental caries owing to their ability to reverse dysbiosis and restore a healthy microbiota. Here, we identified <i>Burkholderia ambifaria</i> AFS098024 as a probiotic candidate isolated from plants.<h4>Methods</h4>The safety of <i>B. ambifaria</i> was evaluated by hemolytic activity, D-lactic acid production and antibiotic susceptibility. <i>In vitro</i> biofilm model derived from the saliva of caries-free and caries-active donors and <i>in vivo</i> rat caries model were used to assess the efficacy of <i>B. ambifaria</i> in caries prevention and treatment.<h4>Results</h4><i>B. ambifaria</i> was safe as a probiotic candidate and it could integrate with <i>in vitro</i> biofilm model. It significantly reduce2024-01-01T00:00:00Z20242026-07-16T13:16:54.074Z2025-04-04T18:54:01.629ZS-EPMC115366933950219010.1080/20002297.2024.2420612