biostudies-literatureChen RFundamental Research Funds for the Central UniversitiesNational Key R&D Program of ChinaResearch Project of Jinan Microecological Biomedicine Shandong LaboratoryShandong Provincial Laboratory ProjectCAMS Innovation Fund for Medical SciencesNational Natural Science Foundation of ChinaHenan Provincial Science and Technology Research Project26817https://www.ebi.ac.uk/biostudies/studies/S-EPMC11538481biostudies-literatureUnknown14(1)The increasing prevalence of carbapenem-resistant Enterobacter cloacae complex (CREC) poses great challenges to infection treatment in the clinical setting. In this study, we reported the emergence of carbapenemase in a rare species, Enterobacter chuandaensis, belonging to the Enterobacter cloacae complex (ECC). We elucidated the genetic characteristics of carbapenem-resistant isolate FAHZZU5885, co-harboring bla<sub>NDM-1</sub> and bla<sub>KPC-2</sub>. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and average nucleotide identity (ANI) analysis were used to identify E. chuandaensis. S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) and Southern blotting were used to clarify the number and size of the plasmids in FAHZZU5885. Antimicrobial pScientific reportsFirst documentation of a clinical multidrug-resistant Enterobacter chuandaensis ST2493 isolate co-harboring bla<sub>NDM-1</sub> and two bla<sub>KPC-2</sub> bearing plasmids.PMC115384812020YFE0204300SYS20220223210231017682072314JNL-2022011B2019-I2M-5-0452022ZFJH003Liu XLiu YLi CGuo XGe HLiu RXu HShen YChen RQiao JFang LfalseFirst documentation of a clinical multidrug-resistant Enterobacter chuandaensis ST2493 isolate co-harboring bla<sub>NDM-1</sub> and two bla<sub>KPC-2</sub> bearing plasmids.The increasing prevalence of carbapenem-resistant Enterobacter cloacae complex (CREC) poses great challenges to infection treatment in the clinical setting. In this study, we reported the emergence of carbapenemase in a rare species, Enterobacter chuandaensis, belonging to the Enterobacter cloacae complex (ECC). We elucidated the genetic characteristics of carbapenem-resistant isolate FAHZZU5885, co-harboring bla<sub>NDM-1</sub> and bla<sub>KPC-2</sub>. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and average nucleotide identity (ANI) analysis were used to identify E. chuandaensis. S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) and Southern blotting were used to clarify the number and size of the plasmids in FAHZZU5885. Antimicrobial p2024-01-01T00:00:00Z2024 Nov2026-06-01T17:41:32.825Z2025-04-06T18:26:55.542ZS-EPMC115384813950096610.1038/s41598-024-78163-2