{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Deichsel S"],"funding":["Deutsche Forschungsgemeinschaft","Universität Hohenheim","NIH HHS"],"pagination":["RP89582"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11540305"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12"],"pubmed_abstract":["Notch signalling activity regulates hematopoiesis in <i>Drosophila</i> and vertebrates alike. Parasitoid wasp infestation of <i>Drosophila</i> larvae, however, requires a timely downregulation of Notch activity to allow the formation of encapsulation-active blood cells. Here, we show that the <i>Drosophila</i> CSL transcription factor Suppressor of Hairless [Su(H)] is phosphorylated at Serine 269 in response to parasitoid wasp infestation. As this phosphorylation interferes with the DNA binding of Su(H), it reversibly precludes its activity. Accordingly, phospho-deficient <i>Su(H)<sup>S269A</sup></i> mutants are immune-compromised. A screen for kinases involved in Su(H) phosphorylation identified Pkc53E, required for normal hematopoiesis as well as for parasitoid immune response. Genetic a"],"journal":["eLife"],"pubmed_title":["Inhibition of the Notch signal transducer CSL by Pkc53E-mediated phosphorylation to fend off parasitic immune challenge in <i>Drosophila</i>."],"pmcid":["PMC11540305"],"funding_grant_id":["P40 OD010949","P40 OD018537","NA 427/5-1"],"pubmed_authors":["Deichsel S","Mastel H","Gahr BM","Fechner J","Nagel AC","Zimmermann M","Preiss A","Frankenreiter L","Preis I"],"additional_accession":[]},"is_claimable":false,"name":"Inhibition of the Notch signal transducer CSL by Pkc53E-mediated phosphorylation to fend off parasitic immune challenge in <i>Drosophila</i>.","description":"Notch signalling activity regulates hematopoiesis in <i>Drosophila</i> and vertebrates alike. Parasitoid wasp infestation of <i>Drosophila</i> larvae, however, requires a timely downregulation of Notch activity to allow the formation of encapsulation-active blood cells. Here, we show that the <i>Drosophila</i> CSL transcription factor Suppressor of Hairless [Su(H)] is phosphorylated at Serine 269 in response to parasitoid wasp infestation. As this phosphorylation interferes with the DNA binding of Su(H), it reversibly precludes its activity. Accordingly, phospho-deficient <i>Su(H)<sup>S269A</sup></i> mutants are immune-compromised. A screen for kinases involved in Su(H) phosphorylation identified Pkc53E, required for normal hematopoiesis as well as for parasitoid immune response. Genetic a","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-06-01T17:33:32.437Z","creation":"2025-04-19T04:24:43.641Z"},"accession":"S-EPMC11540305","cross_references":{"pubmed":["39503739"],"doi":["10.7554/eLife.89582"]}}