biostudies-literatureGuizar Amador MFSenacytUniversidad de Costa RicaDavid and Lucile Packard Foundationjkae211https://www.ebi.ac.uk/biostudies/studies/S-EPMC11540329biostudies-literatureUnknown14(11)Orchidaceae is one of the most prominent flowering plant families, with many species exhibiting highly specialized reproductive and ecological adaptations. An estimated 10% of orchid species in the American tropics are pollinated by scent-collecting male euglossine bees; however, to date, there are no published genomes of species within this pollination syndrome. In this study, we present the first draft genome of an epiphytic orchid from the genus Gongora, a representative of the male euglossine bee-pollinated subtribe Stanhopeinae. The 1.83-Gb de novo genome with a scaffold N50 of 1.7 Mb was assembled using short- and long-read sequencing and chromosome capture (Hi-C) information. Over 17,000 genes were annotated, and 82.95% of the genome was identified as repetitive content. FurthermoreG3 (Bethesda, Md.)The Gongora gibba genome assembly provides new insights into the evolution of floral scent in male euglossine bee-pollinated orchids.PMC11540329APY-NI-2017-05C4074Ramirez SRBogarin DDarragh KSerracin ZDean CLiu JWGuizar Amador MFPerez-Escobar OAPupulin FfalseThe Gongora gibba genome assembly provides new insights into the evolution of floral scent in male euglossine bee-pollinated orchids.Orchidaceae is one of the most prominent flowering plant families, with many species exhibiting highly specialized reproductive and ecological adaptations. An estimated 10% of orchid species in the American tropics are pollinated by scent-collecting male euglossine bees; however, to date, there are no published genomes of species within this pollination syndrome. In this study, we present the first draft genome of an epiphytic orchid from the genus Gongora, a representative of the male euglossine bee-pollinated subtribe Stanhopeinae. The 1.83-Gb de novo genome with a scaffold N50 of 1.7 Mb was assembled using short- and long-read sequencing and chromosome capture (Hi-C) information. Over 17,000 genes were annotated, and 82.95% of the genome was identified as repetitive content. Furthermore2024-01-01T00:00:00Z2024 Nov2026-04-08T19:29:41.608Z2025-04-04T02:34:37.406ZS-EPMC115403293923100610.1093/g3journal/jkae211