biostudies-literatureUnknown14Lau CL<h4>Background</h4>Antimicrobial resistance (AMR) can lead to fatal consequences. AMR genes carriage by phenotypically susceptible bacteria, such as Extended-Spectrum β-Lactamases (ESBL)s in <i>Enterobacteriaceae</i>, have potential implications for AMR spread and therapeutic outcomes. This phenomenon should be investigated.<h4>Methods</h4>Positive blood cultures from hospitalized patients in a Malaysian tertiary center between April 2022 and March 2023 were reviewed. A total of 137 clinical isolates of <i>Escherichia coli (E.coli</i>), <i>Klebsiella pneumoniae</i> (<i>K.pneumoniae</i>), and <i>Klebsiella oxytoca</i> were included. The antibiotic susceptibility and ESBL phenotypes were determined by disk diffusion method and the identification of genotypes by multiplex polymerase chain reaFrontiers in cellular and infection microbiology1429830https://www.ebi.ac.uk/biostudies/studies/S-EPMC11540778biostudies-literaturePrevalence and clinical significance of the genotypic carriage among ESBL phenotype-negative <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> clinical isolates in bacteremia: a study in a Malaysian tertiary center.PMC11540778Naina Mohamed ILau CLTan TLPeriyasamy PRamli RTg Abu Bakar Sidik TMINeoh HMfalsePrevalence and clinical significance of the genotypic carriage among ESBL phenotype-negative <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> clinical isolates in bacteremia: a study in a Malaysian tertiary center.<h4>Background</h4>Antimicrobial resistance (AMR) can lead to fatal consequences. AMR genes carriage by phenotypically susceptible bacteria, such as Extended-Spectrum β-Lactamases (ESBL)s in <i>Enterobacteriaceae</i>, have potential implications for AMR spread and therapeutic outcomes. This phenomenon should be investigated.<h4>Methods</h4>Positive blood cultures from hospitalized patients in a Malaysian tertiary center between April 2022 and March 2023 were reviewed. A total of 137 clinical isolates of <i>Escherichia coli (E.coli</i>), <i>Klebsiella pneumoniae</i> (<i>K.pneumoniae</i>), and <i>Klebsiella oxytoca</i> were included. The antibiotic susceptibility and ESBL phenotypes were determined by disk diffusion method and the identification of genotypes by multiplex polymerase chain rea2024-01-01T00:00:00Z20242026-07-16T20:17:42.892Z2025-04-19T07:41:08.587ZS-EPMC115407783951259010.3389/fcimb.2024.1429830