biostudies-literaturePeltrini RCommunity of Analytical Measurement Science (CAMS UK)Medical Research Council and Engineering & Physical Sciences Research CouncilMedical Research Council and Engineering & Physical Sciences Research Council (Molecular Pathology Node)Leicester NIHR Biomedical Research CentreImperial NIHR Biomedical Research CenterCommunity of Analytical Measurement Science1101-1112https://www.ebi.ac.uk/biostudies/studies/S-EPMC11544360biostudies-literatureUnknown210(9)Rationale: Volatile organic compounds (VOCs) in asthmatic breath may be associated with sputum eosinophilia. We developed a volatile biomarker signature to predict sputum eosinophilia in asthma. Methods: VOCs emitted into the space above sputum samples (headspace) from patients with severe asthma (n = 36) were collected onto sorbent tubes and analyzed using thermal desorption gas chromatography-mass spectrometry (GC-MS). Elastic net regression identified stable VOCs associated with sputum eosinophilia ⩾ 3% and generated a volatile biomarker signature. This VOC signature was validated in breath samples from: 1) patients with acute asthma according to blood eosinophilia ⩾0.3 × 109cells/L or sputum eosinophilia of ⩾3% in the UK EMBER (East Midlands BreatAmerican journal of respiratory and critical care medicineDiscovery and Validation of a Volatile Signature of Eosinophilic Airway Inflammation in Asthma.PMC11544360MR/N005880/1Respiratory themeHedlin GBakke PWilson SPennazza GJames AHeaney LYasinska VGaillard ENehrig JMansoor SSkipp PJKonradsen JCaruso MLazic ZGolbach ECornacchini SBRenwick LHaitchi HMWheelock AWheelock CBrinkman PAdcock IMRichardson MSantonico MWalker SPostle TColeman CReynolds JCPlatt ANizankowska EChanez PSandstrom TAndersson LBourke AMIbrahim Wthe EMBER EPSRC/MRC Consortium and the U-BIOPRED-IMI Study GroupKearley JDjukanovic RFrey USinger FKots MMaitland van der Zee AHIbrahim MKrug NStaudinger HStefanuto PHCordell RLShaw DAlving KPeltrini RZounemat-Kermani NSkrgat SOstling JBaribaud FAffleck KSchleich FHashimoto SThomas PQuek EPowell PHigenbottam TSousa AZhou HZounemat Kermani NZhao Bvan den Heuvel MClokie MRJSingapuri ASoderhall CLouis RBrightling CArmin BMonks PBush AHowarth POlin ACBansal AChung FBarer MRKhokhlovich ERoberts AClarke GWagener ABadi YKolmert JAung HDahlen SELe Quesne JBel EHDDe Meulder BBrandsma JFree RCRoberts GMuton RSiddiqui SHorvath IKFowler SPenate Gomez DPalkonen SMurray CMusial JDanen CSterk PJAbuhelal SGovoni MSun KRao NRaffaele AWilde MFaner RShaw JAUddinCorfield JTenselius OYang FCompton CGeiser TEzerskyte MSamani NJHohlfeld JRupani HSalman DGreening NRomagosa SAgusti AGuo YKAuffray CMontuschi PSuzuki TNordlund Bde Sousa DDahlen BHou RHargadon BAzim ASigmund RKnowles RLoza MJSalas MVersi ANeedham KDamera GCsoma ZCordell RNg LHorvath IDaVeiga Sde Vries RPoinasamy KXavier JSchofieldHekking PWGoodhall AHSupple DVestbo JCoats TPandya HCWikstrom Jonsson ESparreman Mikus MOstridge KOlsson HKLouise J FMjosberg JMumby SMaier DWarren LSpruit MfalseDiscovery and Validation of a Volatile Signature of Eosinophilic Airway Inflammation in Asthma.Rationale: Volatile organic compounds (VOCs) in asthmatic breath may be associated with sputum eosinophilia. We developed a volatile biomarker signature to predict sputum eosinophilia in asthma. Methods: VOCs emitted into the space above sputum samples (headspace) from patients with severe asthma (n = 36) were collected onto sorbent tubes and analyzed using thermal desorption gas chromatography-mass spectrometry (GC-MS). Elastic net regression identified stable VOCs associated with sputum eosinophilia ⩾ 3% and generated a volatile biomarker signature. This VOC signature was validated in breath samples from: 1) patients with acute asthma according to blood eosinophilia ⩾0.3 × 109cells/L or sputum eosinophilia of ⩾3% in the UK EMBER (East Midlands Breat2024-01-01T00:00:00Z2024 Nov2026-06-05T07:46:30.41Z2026-05-14T03:12:18.393ZS-EPMC115443603882012310.1164/rccm.202310-1759OC10.1164/rccm.202310-1759oc