biostudies-literatureWu JFundamental Research Funds for the Central UniversitiesNatural Science Foundation of ChongqingNational Center of Technology Innovation for Pigs3103https://www.ebi.ac.uk/biostudies/studies/S-EPMC11544765biostudies-literatureUnknown14(21)In 2022, a significant PRV outbreak in a southwestern China pig farm led to a high incidence of sow abortion. A serological analysis using <i>gE</i> antigen-based ELISA revealed a high prevalence (69.30%) of PRV <i>gE</i> antibodies among the affected pigs, with a significant variation across different pig populations (1.11-76.12%). We collected additional 5552 pig serum samples and 580 pig cerebrospinal fluid (CSF) samples from various pig farms in Southwest China between 2022 and 2024. The seropositive rates for PRV <i>gE</i> antibodies ranged from 2.36% and 8.65% in the serum samples, while the positive detection rates for the PRV <i>gE</i> gene in the cerebrospinal fluid samples, as determined by PCR, were between 1.06% and 2.36%. The PCR analysis and sequencing of the PRV <i>gB</i>, <Animals : an open access journal from MDPIPrevalence and Genetic Variation Investigation of the Pseudorabies Virus in Southwest China.PMC11544765SWU-KR22036CSTB2024NSCQ-MSX0467NCTIP-XD/C09Wu JZhu JZhang JYan NLuo YYang RFu LWang XZhang LJia MWang YZhou JfalsePrevalence and Genetic Variation Investigation of the Pseudorabies Virus in Southwest China.In 2022, a significant PRV outbreak in a southwestern China pig farm led to a high incidence of sow abortion. A serological analysis using <i>gE</i> antigen-based ELISA revealed a high prevalence (69.30%) of PRV <i>gE</i> antibodies among the affected pigs, with a significant variation across different pig populations (1.11-76.12%). We collected additional 5552 pig serum samples and 580 pig cerebrospinal fluid (CSF) samples from various pig farms in Southwest China between 2022 and 2024. The seropositive rates for PRV <i>gE</i> antibodies ranged from 2.36% and 8.65% in the serum samples, while the positive detection rates for the PRV <i>gE</i> gene in the cerebrospinal fluid samples, as determined by PCR, were between 1.06% and 2.36%. The PCR analysis and sequencing of the PRV <i>gB</i>, <2024-01-01T00:00:00Z2024 Oct2026-05-26T20:04:18.491Z2025-04-04T23:32:30.637ZS-EPMC115447653951882610.3390/ani14213103