{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang X"],"funding":["the Fundamental Research Funds for the Central Universities","the Natural Science Foundation of Chongqing, China","Hunan province college students research learning and innovative experiment project","Shandong & Chongqing Science and technology cooperation project","the National Natural Science Foundation of China","Hunan Provincial Health Commission Project"],"pagination":["1798"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11545376"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(21)"],"pubmed_abstract":["Background
Ubiquitination is an important post-transcriptional modification crucial for maintaining cell homeostasis. As a deubiquitination enzyme, ubiquitin-specific protease 1 (USP1) is associated with tumor progression; however, its role in bladder cancer is unknown. This study aimed to analyze USP1 expression and study its roles in bladder cancer.Methods
The web server GEPIA was used to analyze the USP1 expression. To explore USP1's function in bladder cancer, we constructed USP1-knockout cell lines in UMUC3 cells. A FLAG-USP1 (WT USP1) plasmid and a plasmid FLAG-USP1 C90S (catalytic-inactive mutant) were used to overexpress USP1 in T24 cells. CCK8, colony formation, and Transwell assays were used to assess cell viability, proliferation, and migration. RNA-sequencing (R"],"journal":["Cells"],"pubmed_title":["Ubiquitin-Specific Protease 1 Promotes Bladder Cancer Progression by Stabilizing c-MYC."],"pmcid":["PMC11545376"],"funding_grant_id":["CSTB2023TIAD-LDX0029","202202084830","CSTB2022NSCQMSX0832","S202410542176","81902844，32270760","2021CDJYGRH-004"],"pubmed_authors":["Zhang AQ","Zhang HH","Bao LW","Yu B","Lei J","Li T","Li SZ","Zhang X","Peng P"],"additional_accession":[]},"is_claimable":false,"name":"Ubiquitin-Specific Protease 1 Promotes Bladder Cancer Progression by Stabilizing c-MYC.","description":"Background
Ubiquitination is an important post-transcriptional modification crucial for maintaining cell homeostasis. As a deubiquitination enzyme, ubiquitin-specific protease 1 (USP1) is associated with tumor progression; however, its role in bladder cancer is unknown. This study aimed to analyze USP1 expression and study its roles in bladder cancer.Methods
The web server GEPIA was used to analyze the USP1 expression. To explore USP1's function in bladder cancer, we constructed USP1-knockout cell lines in UMUC3 cells. A FLAG-USP1 (WT USP1) plasmid and a plasmid FLAG-USP1 C90S (catalytic-inactive mutant) were used to overexpress USP1 in T24 cells. CCK8, colony formation, and Transwell assays were used to assess cell viability, proliferation, and migration. RNA-sequencing (R","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2025-04-04T14:52:12.583Z","creation":"2025-04-04T14:52:12.583Z"},"accession":"S-EPMC11545376","cross_references":{"pubmed":["39513905"],"doi":["10.3390/cells13211798"]}}