biostudies-literatureZhang Xthe Fundamental Research Funds for the Central Universitiesthe Natural Science Foundation of Chongqing, ChinaHunan province college students research learning and innovative experiment projectShandong & Chongqing Science and technology cooperation projectthe National Natural Science Foundation of ChinaHunan Provincial Health Commission Project1798https://www.ebi.ac.uk/biostudies/studies/S-EPMC11545376biostudies-literatureUnknown13(21)<h4>Background</h4>Ubiquitination is an important post-transcriptional modification crucial for maintaining cell homeostasis. As a deubiquitination enzyme, ubiquitin-specific protease 1 (USP1) is associated with tumor progression; however, its role in bladder cancer is unknown. This study aimed to analyze USP1 expression and study its roles in bladder cancer.<h4>Methods</h4>The web server GEPIA was used to analyze the USP1 expression. To explore USP1's function in bladder cancer, we constructed USP1-knockout cell lines in UMUC3 cells. A FLAG-USP1 (WT USP1) plasmid and a plasmid FLAG-USP1 C90S (catalytic-inactive mutant) were used to overexpress USP1 in T24 cells. CCK8, colony formation, and Transwell assays were used to assess cell viability, proliferation, and migration. RNA-sequencing (RCellsUbiquitin-Specific Protease 1 Promotes Bladder Cancer Progression by Stabilizing c-MYC.PMC11545376CSTB2023TIAD-LDX0029202202084830CSTB2022NSCQMSX0832S20241054217681902844，322707602021CDJYGRH-004Zhang AQZhang HHBao LWYu BLei JLi TLi SZZhang XPeng PfalseUbiquitin-Specific Protease 1 Promotes Bladder Cancer Progression by Stabilizing c-MYC.<h4>Background</h4>Ubiquitination is an important post-transcriptional modification crucial for maintaining cell homeostasis. As a deubiquitination enzyme, ubiquitin-specific protease 1 (USP1) is associated with tumor progression; however, its role in bladder cancer is unknown. This study aimed to analyze USP1 expression and study its roles in bladder cancer.<h4>Methods</h4>The web server GEPIA was used to analyze the USP1 expression. To explore USP1's function in bladder cancer, we constructed USP1-knockout cell lines in UMUC3 cells. A FLAG-USP1 (WT USP1) plasmid and a plasmid FLAG-USP1 C90S (catalytic-inactive mutant) were used to overexpress USP1 in T24 cells. CCK8, colony formation, and Transwell assays were used to assess cell viability, proliferation, and migration. RNA-sequencing (R2024-01-01T00:00:00Z2024 Oct2025-04-04T14:52:12.583Z2025-04-04T14:52:12.583ZS-EPMC115453763951390510.3390/cells13211798