biostudies-literatureDuong VTNIDDK NIH HHSNCI NIH HHS2401952https://www.ebi.ac.uk/biostudies/studies/S-EPMC11546089biostudies-literatureUnknown34(36)Decellularized small intestine submucosa (dSIS) is a promising biomaterial for promoting tissue regeneration. Isolated from the submucosal layer of animal jejunum, SIS is rich in extracellular matrix (ECM) proteins, including collagen, laminin, and fibronectin. Following mild decellularization, dSIS becomes an acellular matrix that supports cell adhesion, proliferation, and differentiation. Conventional dSIS matrix is usually obtained by thermal crosslinking, which yields a soft scaffold with low stability. To address these challenges, dSIS has been modified with methacrylate groups for photocrosslinking into stable hydrogels. However, dSIS has not been modified with clickable handles for orthogonal crosslinking. Here, we report the development of norbornene-modified dSIS, named dSIS-NB, vAdvanced functional materialsPhoto-responsive decellularized small intestine submucosa hydrogels.PMC11546089R01 DK127436R01 CA227737Luong NHLin CCDuong VTChang CYNguyen HDfalsePhoto-responsive decellularized small intestine submucosa hydrogels.Decellularized small intestine submucosa (dSIS) is a promising biomaterial for promoting tissue regeneration. Isolated from the submucosal layer of animal jejunum, SIS is rich in extracellular matrix (ECM) proteins, including collagen, laminin, and fibronectin. Following mild decellularization, dSIS becomes an acellular matrix that supports cell adhesion, proliferation, and differentiation. Conventional dSIS matrix is usually obtained by thermal crosslinking, which yields a soft scaffold with low stability. To address these challenges, dSIS has been modified with methacrylate groups for photocrosslinking into stable hydrogels. However, dSIS has not been modified with clickable handles for orthogonal crosslinking. Here, we report the development of norbornene-modified dSIS, named dSIS-NB, v2024-01-01T00:00:00Z2024 Sep2026-05-29T18:46:00.741Z2026-04-08T05:43:16.511ZS-EPMC115460893952528810.1002/adfm.202401952