{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Lou J"],"pubmed_abstract":["Background
Breast cancer (BC) is a serious health threat to the patients. The present work explored the mechanism of miR-301b-3p and transforming growth factor-beta receptor 2 (TGFBR2 ) in affecting BC progression.Methods
The miR-301b-3p-inhibitor and si-TGFBR2 solution were added to the DEME/F12 medium to culture the BC and normal breast epithelial cell lines to prepare negative control, miR-301b-3p-IN and miR-301b-3p-IN+si-TGFBR2 in the two types of cell lines. The relative expression of target genes and the interference effect were analyzed by quantitative real-time PCR (qRT- PCR). Cell viability was detected applying cell counting kit-8 (CCK-8) assay. Transwell and wound healing assay were conducted to evaluate the invasion and migration of BC cells after miR-301b-3p in"],"journal":["PeerJ"],"pagination":["e18324"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11546148"],"repository":["biostudies-literature"],"pubmed_title":["MiR-301b-3p promotes breast cancer development through inhibiting the expression of transforming growth factor-beta receptor 2."],"pmcid":["PMC11546148"],"pubmed_authors":["Liu X","Wu M","Xie Y","Mao W","Ying X","Lou J"],"additional_accession":[]},"is_claimable":false,"name":"MiR-301b-3p promotes breast cancer development through inhibiting the expression of transforming growth factor-beta receptor 2.","description":"Background
Breast cancer (BC) is a serious health threat to the patients. The present work explored the mechanism of miR-301b-3p and transforming growth factor-beta receptor 2 (TGFBR2 ) in affecting BC progression.Methods
The miR-301b-3p-inhibitor and si-TGFBR2 solution were added to the DEME/F12 medium to culture the BC and normal breast epithelial cell lines to prepare negative control, miR-301b-3p-IN and miR-301b-3p-IN+si-TGFBR2 in the two types of cell lines. The relative expression of target genes and the interference effect were analyzed by quantitative real-time PCR (qRT- PCR). Cell viability was detected applying cell counting kit-8 (CCK-8) assay. Transwell and wound healing assay were conducted to evaluate the invasion and migration of BC cells after miR-301b-3p in","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024","modification":"2026-07-09T13:19:59.77Z","creation":"2025-04-06T11:47:39.968Z"},"accession":"S-EPMC11546148","cross_references":{"pubmed":["39525474"],"doi":["10.7717/peerj.18324"]}}