biostudies-literatureUnknown12Lou J<h4>Background</h4>Breast cancer (BC) is a serious health threat to the patients. The present work explored the mechanism of miR-301b-3p and transforming growth factor-beta receptor 2 (TGFBR2 ) in affecting BC progression.<h4>Methods</h4>The miR-301b-3p-inhibitor and si-TGFBR2 solution were added to the DEME/F12 medium to culture the BC and normal breast epithelial cell lines to prepare negative control, miR-301b-3p-IN and miR-301b-3p-IN+si-TGFBR2 in the two types of cell lines. The relative expression of target genes and the interference effect were analyzed by quantitative real-time PCR (qRT- PCR). Cell viability was detected applying cell counting kit-8 (CCK-8) assay. Transwell and wound healing assay were conducted to evaluate the invasion and migration of BC cells after miR-301b-3p inPeerJe18324https://www.ebi.ac.uk/biostudies/studies/S-EPMC11546148biostudies-literatureMiR-301b-3p promotes breast cancer development through inhibiting the expression of transforming growth factor-beta receptor 2.PMC11546148Liu XWu MXie YMao WYing XLou JfalseMiR-301b-3p promotes breast cancer development through inhibiting the expression of transforming growth factor-beta receptor 2.<h4>Background</h4>Breast cancer (BC) is a serious health threat to the patients. The present work explored the mechanism of miR-301b-3p and transforming growth factor-beta receptor 2 (TGFBR2 ) in affecting BC progression.<h4>Methods</h4>The miR-301b-3p-inhibitor and si-TGFBR2 solution were added to the DEME/F12 medium to culture the BC and normal breast epithelial cell lines to prepare negative control, miR-301b-3p-IN and miR-301b-3p-IN+si-TGFBR2 in the two types of cell lines. The relative expression of target genes and the interference effect were analyzed by quantitative real-time PCR (qRT- PCR). Cell viability was detected applying cell counting kit-8 (CCK-8) assay. Transwell and wound healing assay were conducted to evaluate the invasion and migration of BC cells after miR-301b-3p in2024-01-01T00:00:00Z20242026-07-09T13:19:59.77Z2025-04-06T11:47:39.968ZS-EPMC115461483952547410.7717/peerj.18324