biostudies-literatureKruger JCarsten Bender-Leukämie-StiftungWerner-Otto-FoundationMildred Scheel Nachwuchszentrum11412https://www.ebi.ac.uk/biostudies/studies/S-EPMC11546357biostudies-literatureUnknown25(21)Comprehensive characterization of AML-associated T cells during disease progression is essential to identify relevant immune escape mechanisms and new immunotherapeutic approaches. Investigating the processes that lead to an immunosuppressive environment under progression of AML is difficult in humans, because by the time of diagnosis the disease is often progressed far beyond the initial stages. Therefore, to investigate T-cell phenotypes during progression a C57BL/6 mouse model was used. The CD3⁺ T cells were characterized by performing multiparametric flow analyses at different time points (day 0 = healthy mice, day 7, day 14, and day 21). The study revealed that the spleen is highly infiltrated by reg CD4⁺ T cells at day 21 of AML progression. These spleen-infiltrInternational journal of molecular sciencesMurine Regulatory CD4<sup>+</sup> T Cells Are Increased in Leukemic Spleens and Show High Co-Expression of Co-Regulatory Molecules CD39, CD73, PD1, and TIGIT.PMC11546357n.a.Kruger JMuschhammer JBokemeyer CFiedler WWellbrock JKruppa NModemann FBehrmann LWitt MBrauneck FfalseMurine Regulatory CD4<sup>+</sup> T Cells Are Increased in Leukemic Spleens and Show High Co-Expression of Co-Regulatory Molecules CD39, CD73, PD1, and TIGIT.Comprehensive characterization of AML-associated T cells during disease progression is essential to identify relevant immune escape mechanisms and new immunotherapeutic approaches. Investigating the processes that lead to an immunosuppressive environment under progression of AML is difficult in humans, because by the time of diagnosis the disease is often progressed far beyond the initial stages. Therefore, to investigate T-cell phenotypes during progression a C57BL/6 mouse model was used. The CD3⁺ T cells were characterized by performing multiparametric flow analyses at different time points (day 0 = healthy mice, day 7, day 14, and day 21). The study revealed that the spleen is highly infiltrated by reg CD4⁺ T cells at day 21 of AML progression. These spleen-infiltr2024-01-01T00:00:00Z2024 Oct2025-04-22T19:42:21.228Z2025-04-06T02:53:36.719ZS-EPMC115463573951896910.3390/ijms252111412