biostudies-literatureSultana SUniversity of Western Australia5161https://www.ebi.ac.uk/biostudies/studies/S-EPMC11547264biostudies-literatureUnknown29(21)This is the first study to report on high performance thin layer chromatography (HPTLC) generated spectrophotometric data to systematically capture flavonoid compounds using optimized derivatization with either AlCl₃ or NaNO₂-AlCl₃-NaOH as visualisation reagents. While the traditional AlCl₃ colorimetric method using UV-Vis analysis provides valuable insights into the presence of flavonoids and allows derivation of the total flavonoid content (TFC) of a sample, HPTLC fingerprints obtained after spraying with AlCl₃ or NaNO₂-AlCl₃-NaOH enable the visualization of the various flavonoids present in a sample based on their respective absorption shifts, thus complementing the traditional TFC assay. In this study, 40 diffMolecules (Basel, Switzerland)Investigating Flavonoids by HPTLC Analysis Using Aluminium Chloride as Derivatization Reagent.PMC11547264N/AFoster KJHossain MLLim LYSultana SLocher CSostaric TfalseInvestigating Flavonoids by HPTLC Analysis Using Aluminium Chloride as Derivatization Reagent.This is the first study to report on high performance thin layer chromatography (HPTLC) generated spectrophotometric data to systematically capture flavonoid compounds using optimized derivatization with either AlCl₃ or NaNO₂-AlCl₃-NaOH as visualisation reagents. While the traditional AlCl₃ colorimetric method using UV-Vis analysis provides valuable insights into the presence of flavonoids and allows derivation of the total flavonoid content (TFC) of a sample, HPTLC fingerprints obtained after spraying with AlCl₃ or NaNO₂-AlCl₃-NaOH enable the visualization of the various flavonoids present in a sample based on their respective absorption shifts, thus complementing the traditional TFC assay. In this study, 40 diff2024-01-01T00:00:00Z2024 Oct2025-04-04T00:38:50.817Z2025-04-04T00:38:50.817ZS-EPMC115472643951980210.3390/molecules29215161