biostudies-literatureUnknown29(21)Jessy Mercy DWound healing is a complex process that can be improved through advanced biomedical approaches. Incorporating nanopolymers and plant extracts into wound dressings offers a favorable strategy for promoting tissue repair. Nanopolymers provide a controlled environment for sustained drug release while also protecting the wound from external contaminants. When combined with bioactive compounds from plant extracts, which possess antioxidant, anti-inflammatory, and antimicrobial properties, this hybrid approach can accelerate healing, reduce infection, and improve tissue regeneration. Hence, in this study, we have synthesized alginate/gelatin hydrogel blended with only nanosilver (Alg/gel-Ag) and with nanosilver and plant extracts like aloe vera, curcumin, plantain peel extract, and <i>Calendula</i> flower petal extract (Alg/gel-AgP). The synthesized hydrogels were characterized using different photophysical tools, and the cytotoxicity effect was studied using a fibroblast cell line (V79). The antibacterial effect of the hydrogels was also observed against <i>E. coli</i> and <i>S. aureus</i>, determining the MIC and MBC. The wound healing in vitro was also assessed using scratch assay which depicted a rapid wound closure for Alg/gel-AgP compared to the untreated control and Alg/gel-Ag. The combined effect between nanotechnology and natural extracts represents a novel and effective approach for enhancing the wound healing process.Molecules (Basel, Switzerland)5004https://www.ebi.ac.uk/biostudies/studies/S-EPMC11547294biostudies-literatureEnhancing Wound Healing with Nanohydrogel-Entrapped Plant Extracts and Nanosilver: An In Vitro Investigation.PMC11547294Udayakumar SGirigoswami ADeepika BThirumalai AJessy Mercy DJanani GGirigoswami KfalseEnhancing Wound Healing with Nanohydrogel-Entrapped Plant Extracts and Nanosilver: An In Vitro Investigation.Wound healing is a complex process that can be improved through advanced biomedical approaches. Incorporating nanopolymers and plant extracts into wound dressings offers a favorable strategy for promoting tissue repair. Nanopolymers provide a controlled environment for sustained drug release while also protecting the wound from external contaminants. When combined with bioactive compounds from plant extracts, which possess antioxidant, anti-inflammatory, and antimicrobial properties, this hybrid approach can accelerate healing, reduce infection, and improve tissue regeneration. Hence, in this study, we have synthesized alginate/gelatin hydrogel blended with only nanosilver (Alg/gel-Ag) and with nanosilver and plant extracts like aloe vera, curcumin, plantain peel extract, and <i>Calendula</i> flower petal extract (Alg/gel-AgP). The synthesized hydrogels were characterized using different photophysical tools, and the cytotoxicity effect was studied using a fibroblast cell line (V79). The antibacterial effect of the hydrogels was also observed against <i>E. coli</i> and <i>S. aureus</i>, determining the MIC and MBC. The wound healing in vitro was also assessed using scratch assay which depicted a rapid wound closure for Alg/gel-AgP compared to the untreated control and Alg/gel-Ag. The combined effect between nanotechnology and natural extracts represents a novel and effective approach for enhancing the wound healing process.2024-01-01T00:00:00Z2024 Oct2025-04-04T18:56:04.726Z2025-04-04T18:56:04.726ZS-EPMC115472943951964610.3390/molecules29215004