biostudies-literatureĐorđevic Zlatkovic MRMinistry of Science, Technological Development and Innovations of the Republic of Serbia3045https://www.ebi.ac.uk/biostudies/studies/S-EPMC11548152biostudies-literatureUnknown13(21)An essential oil dominated by germacrene D (19.3% by GC) was isolated from the fresh fruit of <i>Amorpha fruticosa</i> L. (Fabaceae). Agglomerative clustering and <i>k</i>-means clustering were employed to compare the composition of the oil with the existing literature data, suggesting that the <i>A. fruticosa</i> used in this study represents a new chemotype. The essential oil was evaluated for its antinociceptive activity using the acetic acid-induced writhing test in rats at doses of 400, 200, and 100 mg/kg. All tested doses reduced the number of writhes induced by the intraperitoneal injection of acetic acid. The 400 mg/kg dose of the oil demonstrated a 54.4% inhibition, which was statistically different from the positive control, aspirin, which showed 90.2% inhibition at a dose of 200Plants (Basel, Switzerland)Chemodiversity and Antinociceptive Activity of <i>Amorpha fruticosa</i> L. Essential Oil.PMC11548152451-03-65/2024-03/200124, 451-03-66/2024-03/200124 and 451-03-65/2024-03/200113Radulovic NSStojanovic NMĐorđevic Zlatkovic MRRandjelovic PJZlatkovic DBfalseChemodiversity and Antinociceptive Activity of <i>Amorpha fruticosa</i> L. Essential Oil.An essential oil dominated by germacrene D (19.3% by GC) was isolated from the fresh fruit of <i>Amorpha fruticosa</i> L. (Fabaceae). Agglomerative clustering and <i>k</i>-means clustering were employed to compare the composition of the oil with the existing literature data, suggesting that the <i>A. fruticosa</i> used in this study represents a new chemotype. The essential oil was evaluated for its antinociceptive activity using the acetic acid-induced writhing test in rats at doses of 400, 200, and 100 mg/kg. All tested doses reduced the number of writhes induced by the intraperitoneal injection of acetic acid. The 400 mg/kg dose of the oil demonstrated a 54.4% inhibition, which was statistically different from the positive control, aspirin, which showed 90.2% inhibition at a dose of 2002024-01-01T00:00:00Z2024 Oct2026-07-09T13:25:31.964Z2025-04-07T00:02:05.571ZS-EPMC115481523951996310.3390/plants13213045