biostudies-literatureUnknownTian GT cells are key mediators of alloresponse during liver transplantation (LTx). However, the dynamics of donor-reactive T-cell clones in peripheral blood during a clinical T-cell-mediated rejection (TCMR) episode remain unknown. Here, we collected serial peripheral blood mononuclear cell samples spanning from pre-LTx to 1 year after LTx and available biopsies during the TCMR episodes from 26 rejecting patients, and serial peripheral blood mononuclear cell samples were collected from 96 nonrejectors. Immunophenotypic and repertoire analyses were integrated on T cells from rejectors, and they were longitudinally compared to nonrejected patients. Donor-reactive T-cell clone was identified and tracked by cross-matching with the mappable donor-reactive T-cell receptor repertoire of each donor-recLiver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Societyhttps://www.ebi.ac.uk/biostudies/studies/S-EPMC11548824biostudies-literatureAlloreactive T cells temporarily increased in the peripheral blood of patients before liver allograft rejection.PMC11548824Zhi YQiu WTian GLv GHuang HSong SChen YSun XLi MYu YJiao WfalseAlloreactive T cells temporarily increased in the peripheral blood of patients before liver allograft rejection.T cells are key mediators of alloresponse during liver transplantation (LTx). However, the dynamics of donor-reactive T-cell clones in peripheral blood during a clinical T-cell-mediated rejection (TCMR) episode remain unknown. Here, we collected serial peripheral blood mononuclear cell samples spanning from pre-LTx to 1 year after LTx and available biopsies during the TCMR episodes from 26 rejecting patients, and serial peripheral blood mononuclear cell samples were collected from 96 nonrejectors. Immunophenotypic and repertoire analyses were integrated on T cells from rejectors, and they were longitudinally compared to nonrejected patients. Donor-reactive T-cell clone was identified and tracked by cross-matching with the mappable donor-reactive T-cell receptor repertoire of each donor-rec2024-01-01T00:00:00Z2024 Jun2025-04-05T23:09:55.136Z2025-04-05T23:09:55.136ZS-EPMC115488243890003110.1097/LVT.0000000000000425